DOC-00322-ENA.doc Page 14 of 29 ! Wescor, Inc
CAUTION! Do not subject stainer to freezing temperatures while aqueous fluids remain in any
reagent lines. Serious damage can result.
f. Operating Instruction (8.7a and 8.4j)
i. Initial Instrument Setup
• Install the drain tube
• Plug in the power cord and switch the power on
• Install all reagent bottles
• Prime all reagent lines
• Run the CLEAN cycle twice to purge the reagent nozzles of precipitates and debris. (See Clean Cycle 6.f.ix.)
NOTE: Dirty nozzles cause most staining problems. Check spray pattern and clean nozzles as
necessary.
ii. Select Stain Mode
Press PROG at power up to open the program menu. Press 1 to select Carbol Fuchsin or press 2 to select
Fluorescence.
iii. Load Carousel
Press the center button to remove the carousel lid. Load the 12-slide carousel with labeled end of the slide facing
the outer rim of the carousel. Load the 30-slide carousel with labeled end of the slide facing the carousel hub.
Smears in either carousel must face clockwise
The stainer can be programmed to save reagent when staining partial loads in either the 12-slide or the 30-slide
carousel. To use this feature, place slides in the marked slots on the 12-slide or 30-slide carousel and enter the
number of slides on the keypad. Put the first slide in position 1, the second in position 2, and so on. If there is an
odd number of slides, place a blank slide in the next position for balance. If there are empty slots in the carousel,
place a blank blocking slide in the first empty slot clockwise from a specimen slide and another blank blocking
slide directly across the carousel.
iv. Load Instrument
Replace the carousel lid by pressing the release button while lowering the lid over the indexing posts. Release the
button and press the lid handle lightly until it clicks into place. Place the carousel into the stainer and close the
stainer lid.
v. Program Number of Slides
If staining a partial load, enter the number of slides into the stainer by using the numbered keypads. Slide selection
defaults to Full Carousel at the end of the run or after pressing STOP.
This instrument treats anything over a partial load as a full carousel. To stain more than a partial load there is no
need to program the number of slides. A partial load on the 12-slide carousel is anything from 1 to 6 slides. A
partial load on the 30-slide carousel is anything from 1-16 slides.
vi. Select Intensity
Press PROGRAM, then select Stain Intensity 1 through 6. The selected stain cycle remains current until it is
changed. This enables the running of multiple batches at the same setting without reprogramming.
NOTE: When you power up the stainer, or whenever power is interrupted, programming defaults to the
full carousel option and the last stain settings used, all of which are indicated on the display.
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