DOC-00322-ENA.doc Page 28 of 29 ! Wescor, Inc
If stain begins to appear within 10 seconds (or once an air lock is resolved), prime all reagent lines. This can be done
manually or by using Prime Lines or Volume Test functions. When properly primed, a steady stream of reagent (no
sputtering or breaks) flows from each nozzle receptacle while pressing the corresponding prime button.
WARNING! Never operate a dry pump longer than 10 seconds.
Simultaneously Priming All Lines
Press VOLUME TEST. Press 4 on the keypad to prime all the reagent lines. The pumps run for 60 seconds. After
priming reagent lines, replace the nozzles. Return each nozzle to its original location for consistent staining
performance.
Priming Individual Lines
Prime manually for 60 seconds or press VOLUME TEST. Press 2 on the Keypad. Press the desired prime button.
The pump for that position runs for 20 seconds. Repeat twice more to achieve 60 seconds of flow.
After priming all reagent lines, replace the nozzles. Return each nozzle to its original location for consistent staining
performance.
With the nozzles installed, repeat the priming sequence above. Use a carousel in the instrument. A fine cone of spray
should come from each nozzle. Run the Spray Volume and Spray Pattern tests. After verifying nozzle performance,
run the CLEAN cycle.
vi. Run the CLEAN cycle
The CLEAN cycle is a two-stage process that uses methanol or ethanol to purge the reagent nozzles of precipitates
and debris, then reprimes reagents into the nozzles. Each stage of the cycle requires user input to continue the
process. This allows delaying the second stage indefinitely. The steps described below are for initial setup (see
following Note), routine cleaning of the instrument, or to keep the nozzles clean while the instrument is idle. We
recommend frequent use (at least once per shift) of the CLEAN cycle to ensure reliable nozzle performance.
NOTE: Run the following CLEAN cycle routine twice during initial setup, to help remove any air bubbles
from the reagent lines and ready the instrument for slide staining.
• Place an empty carousel in the instrument and close the lid.
• Push CLEAN. This sprays methanol or ethanol (Reagent E) through the nozzles and onto the carousel. When
the cycle is completed the display reads:
PRESS CLEAN TO REPRIME.
• Wipe nozzle orifices to remove all residual liquid.
• At this point, the instrument “stands by” with methanol or ethanol remaining in the nozzles until pressing
CLEAN again. The instrument can be left in this standby mode for extended periods to prevent clogging while
the stainer is idle.
• If not staining slides immediately, leave the instrument in standby mode until ready to stain. This is particularly
important if the instrument will be idle for more than 4 hours. Later, when ready to stain slides, make sure there
is a carousel in the instrument, then:
• Press CLEAN again. This reprimes reagent into the nozzles, making the instrument ready for staining.
CAUTION! Do not place any carousel loaded with specimens in the instrument while CLEAN TO
REPRIME appears on the display. Specimens will be damaged if they contact excess reagents
sprayed from the nozzles when you press CLEAN.
NOTE: Pressing STOP during the clean or reprime cycles causes a warning to be displayed until a
complete Clean and Reprime cycle is completed.
r. Process for Cleaning, Decontamination (8.7q)
Under normal clinical use the Aerospray
"
AFB Stainer/Cytocentrifuge poses very little risk of biological infection to
laboratory workers. The stainer is essentially an environmental surface, which should be kept clean. Only low-level
disinfection is required.
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