DOC-00322-ENA.doc Page 27 of 29 ! Wescor, Inc
iii. Install all reagent bottles
Place the reagent bottles front to back in the following order:
Carbol Fuchsin (Option 1)
(A) Carbol Fuchsin Decolorizer
(B) Brilliant Green
(C) Kinyoun
(D) Deionized Water
(E) Methanol or Ethanol
Carbol Fuchsin (Option 2)
(A) Carbol Fuchsin Decolorizer
(B) Methylene Blue
(C) Ziehl-Neelsen
(D) Deionized Water
(E) Methanol or Ethanol
Fluorescence (Option 1)
(A) Fluorescence Decolorizer
(B) Potassium Permanganate
(C) Auramine-Rhodamine
(D) Deionized Water
(E) Methanol or Ethanol
Fluorescence (Option 2)
(A) Fluorescence Decolorizer
(B) Potassium Permanganate
(C) Auramine
(D) Deionized Water
(E) Methanol or Ethanol
WARNING! Reagents used in the Aerospray Stainer contain moderately hazardous chemicals that
require care in handling. Always use appropriate safety measures including gloves and eye
protection when handling reagents.
CAUTION! To avoid severe damage, never use reagents containing organic solvents in this
instrument unless they are supplied by Wescor or specified in official Wescor formulation
instructions.
Remove the cap from each bottle. Remove the central portion of the seal.
Insert the dip tubes into the reagent bottles and screw on the ring caps.
iv. Aerospray Nozzle Maintenance Kit
The Aerospray Nozzle Maintenance Kit (AC-075) is provided to help maintain optimum performance from the
reagent delivery system. The kit contains tools and equipment to clean the nozzles, check reagent delivery and to
prime the instrument.
v. Prime all reagent lines
For peak stainer performance, thoroughly purge and prime each reagent delivery line using the following
instructions. This procedure uses approximately 250 mL of reagent per line. Extra reagent is included with the new
instrument for this purpose.
Remove each spray nozzle with the provided nozzle tool by turning it counterclockwise. Note the location of each
nozzle (so you can return it to the original location).
Place a carousel in the stainer to prevent stain from entering the motor shaft. Briefly press each prime button. Stain
should appear within 10 seconds. If no stain appears within 10 seconds there may be an air lock in the line.
Immediately stop priming. Use Reagent Pump Priming Tool (AC-069), part of Nozzle Maintenance Kit, to remove
the air lock.
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