DOC-00322-ENA.doc Page 25 of 29 ! Wescor, Inc
WARNING! Always use hand and eye protection when performing preventive maintenance on this
instrument.
NOTE: If using permanganate in the B reagent line, the B nozzle requires daily cleaning. Maintain a
spare nozzle soaking in nozzle cleaning solution. Use a nozzle cap filled with cleaning solution on the B
nozzle during idle periods.
NOTE: As you begin a new month, the “Volume After Month’s Cleaning” should be entered as the
“Previous Month’s Ending Volume.”
p. Troubleshooting
The system programming controls all stain delivery calibrations. The values of these settings are preset and not
changeable. If you suspect a problem with reagent delivery, diagnose the problem by assessing the performance of
each spray nozzle.
Press the prime buttons and observe each nozzle for uniform dispersion, pattern shape, and direction of spray. Test
spray pattern and spray volume to determine which nozzle is not functioning properly. Use the instructions under
Disassembly and Cleaning to clean any nozzle that does not appear to function normally.
Disassembly and Cleaning
The automatic clean cycle eliminates the evaporative accumulation of reagent solute in the nozzle system. Foreign
material may, however, get into the system and eventually plug the nozzle. If this happens, manually disassemble
and clean the spray nozzle as follows:
1. Slide the nozzle tool over the spray nozzle and turn counter-clockwise to loosen and remove.
2. While taking the nozzle apart, become familiar with the nozzle, its parts and assembly.
NOTE
: The following procedure requires using the Nozzle Maintenance Kit. Use the kit to prevent
mixing nozzles or nozzle parts. When removing Aerospray nozzle(s), be sure to return all nozzles to
their original positions after cleaning. This helps ensure consistent staining performance.
3. Hold the spray nozzle with the nozzle tool and insert the 5/32 nozzle hex wrench into the compression screw.
Turn counter-clockwise to loosen and remove. DO NOT DROP COMPRESSION SCREW OR SWIRL
CONE.
4. Remove the o-ring.
NOTE: Excessive force used to loosen and remove the compression screw can damage the plastic
nozzle tool. If the compression screw cannot be readily loosened, soak the nozzle in methanol, ethanol,
or prepared SS-029C to remove residue. If the problem continues, use light penetrating oil and a 5/8-
inch wrench to loosen the nozzle.
CAUTION! Do not use hardened metal instruments to clean or scrape nozzle components.
5. Place disassembled metal nozzle parts in one of the provided 50 mL centrifuge tubes.
6. Fill tube to 25 mL mark with methanol, ethanol, or prepared SS-029C.
7. Cap tube, agitate, and then soak parts until clean.
WARNING! Always wear protective clothing and eye protection when using the SS-029C.
Dispose of used cleaning solution in the correct manner.
8. Agitate again, and pour off the solution.
9. Use the provided cleaning wire (AC-059) to clean the nozzle orifice.
10. Remove any material in the swirl cone grooves by sliding the edge of a piece of paper through each groove.
11. Inspect nozzle parts to ensure they are completely clean. Repeat soaking if necessary.
NOTE: To prevent mixing nozzle parts, use the tube stand inside the Nozzle Maintenance Kit. Place
tube containing nozzle parts in the punched hole that corresponds with the reagent line that the nozzle
came from.
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