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Zeiss TIRF 3 User Manual

Zeiss TIRF 3
110 pages
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Laser TIRF 3 Imaging System
Operating Manual

Table of Contents

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Zeiss TIRF 3 Specifications

General IconGeneral
BrandZeiss
ModelTIRF 3
CategoryLaboratory Equipment
LanguageEnglish

Summary

DEVICE SAFETY

General Notes

Provides general safety information and standards compliance for the imaging system.

Safety Instructions

Details crucial instructions for safe operation and handling of the laser system.

Installation Instructions

Guidance on proper installation procedures and safety requirements.

Notes on Computer and Software

Information on computer and software safety requirements for system operation.

Service and Customer Interfaces

Details on connection points for service and customer interaction.

Warning and Information Labels on the Instrument

Illustrates and explains warning and information labels on the equipment.

Notes on Warranty

Outlines the manufacturer's warranty terms and conditions.

INSTRUMENT DESCRIPTION

Intended Use

Defines the purpose and application of the Laser TIRF imaging system.

Operating Principle

Explains the scientific principles and working mechanism of TIRF microscopy.

Instrument Description and Main Features

Details the components and key features of the Laser TIRF imaging system.

Laser Bench Tower and Laser Bench Rack or Laser Bench Rack 3F

Details the Laser Bench Tower and Laser Bench Rack components.

Signal Distribution Box

Information on the signal distribution box and its function.

TIRF Controller

Describes the TIRF controller used for system operation.

Laser Safety for the Sample Compartment

Ensures laser safety within the sample compartment.

Basic kit laser safety Rev. 2

Details the basic laser safety kit and its components.

Remote switch for laser mode

Explains the remote switch for controlling laser operation modes.

Laser Safety Concept

Outlines the overall laser safety concept and interlock system.

Configuration Overview of the Laser TIRF Imaging System

Provides an overview of system configuration options and choices.

System Overview Laser TIRF Imaging System

Presents a visual overview of the Laser TIRF imaging system components.

Compatibility of Stages and Laser Safety Devices

Details compatibility between stages and laser safety devices.

Compatibility of XLmulti Incubators and Accessories

Lists compatibility of XLmulti incubators with accessories.

Overview of Stage Versions for TIRF in Axio Observer Program

Summarizes stage versions compatible with Axio Observer TIRF.

Objectives to Be Used with the Laser TIRF Imaging System

Recommends suitable objectives for optimal TIRF performance.

Fluorescence Filter Sets to Be Used with the Laser TIRF Imaging System

Lists suitable fluorescence filter sets for TIRF microscopy.

Combination of Laser TIRF Imaging System and Colibri.2 Illumination System

Details combinations with the Colibri.2 illumination system.

Optical Fibers to Be Used with the Laser TIRF Imaging System

Specifies optical fibers suitable for the TIRF imaging system.

Technical Data

Provides technical specifications for system components.

START-UP

Installation Requirements

Covers requirements for installing the Laser TIRF imaging system.

Space Requirements

Details the physical space and layout needed for system installation.

Power Requirements

Specifies the electrical power needs and connections for the system.

Ambient Conditions

Defines the environmental conditions required for system operation.

Requirements for the Microscope Stand

Specifies requirements for the compatible microscope stand.

Basic Microscope Settings

Guides on essential basic microscope settings for operation.

Mounting the Laser Safety Cover for Reflected Light Provided for Mechanical and Scanning Stages

Instructions for mounting the laser safety cover for specific stages.

Mounting the S TIRF S1/S TIRF DARK S1 Incubator

Step-by-step guide for mounting the S TIRF S1/DARK S1 incubator.

Incubator XLmulti S1 RED LS and Incubator XLmulti S1 DARK LS

Details on the XLmulti S1 incubators and their usage.

Laser Safety: Position of the Reed Contacts

Explains laser safety through reed contact positions within the incubator.

Mounting (XLmulti Incubator)

Provides instructions for mounting the XLmulti S1 incubators.

PM S1 LS Incubators

Details the PM S1 LS incubator and its heating inserts.

Mounting (PM S1 LS Incubator)

Instructions for mounting the PM S1 LS incubator.

Assembly of the Magnet Screw

Guide for assembling the magnet screw for laser safety.

S TIRF S1 and S TIRF DARK S1 Incubators

Information on S TIRF S1 and S TIRF DARK S1 incubators.

Insert Slider Laser TIRF

Procedure for inserting the Slider Laser TIRF into the microscope.

OPERATION

Control and Functional Elements

Overview of the control and functional elements of the system.

Manual Slider Laser TIRF / mot.

Details the control elements of the manual and motorized Slider Laser TIRF.

Laser Bench Tower

Explains the control and functional elements of the Laser Bench Tower.

Laser Bench Rack

Explains the control and functional elements of the Laser Bench Rack.

Switching on

Step-by-step guide for powering on the entire system.

Switching off

Procedure for safely powering off the system and its components.

ZEN Software (blue edition)

Guide to controlling the system via ZEN 2012 software.

Checking and Adjusting the Laser Beam Divergence and the TIRF Angle

Guides on adjusting laser beam divergence and TIRF angle.

Delivery Scope of the TIRF Adjustment Tool Kit

Lists components included in the TIRF adjustment tool kit.

Adjusting the Laser Beam Divergence Using the Adjustment Tool

Detailed steps for adjusting laser beam divergence using the tool.

Adjusting and Checking the TIRF Angle Using the Adjustment Tool

Procedure for adjusting and checking the TIRF angle with the tool.

Adjusting the TIRF Effect on the TIRF Slider man.

Instructions for adjusting the TIRF effect on the manual slider.

Controlling the Laser TIRF Imaging System Using ZEN 2012 (Blue Edition)

Guide to controlling the system via ZEN 2012 software.

Calibration of the Angle Using the Motorized Slider Laser TIRF

Procedure for calibrating the angle on the motorized slider.

Measurement of Penetration Depth

Explains how to measure the penetration depth of the evanescent field.

Snell's Law

Details Snell's Law as applied to TIRF microscopy.

The Angle-Voltage Relationship of the Slider Laser TIRF

Describes the relationship between angle and voltage for the slider.

Determining the Critical Angle

Method for determining the critical angle for TIRF.

Determination of the Refractive Index of the Specimen

Procedure for determining the refractive index of the specimen.

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE

Instrument Care

Guidelines for basic user care and cleaning of the instrument.

Instrument Maintenance

References for detailed instrument maintenance procedures.

Troubleshooting

Provides solutions for common problems encountered during operation.

Fuse Replacement

Instructions for replacing fuses in various system components.

Changing the HAL Halogen Lamp

Procedure for changing the halogen lamp.

Retrofitting the Laser Units

Information on retrofitting laser units by authorized personnel.

Mounting the Camera

Steps for connecting and mounting the camera to the microscope.

Connecting the HXP 120 Illumination Unit

Guide for connecting the HXP 120 illumination unit.

Service Request

Information on how to request service and support.

USING THE SLIDER LASER TIRF WITH CUSTOMERS' OWN LASERS

Laser Safety Concept

Laser safety concept for using external laser modules.

Safety Kit for Customer-Provided Laser Module

Details the safety kit for integrating customer-provided laser modules.

Remote switch for laser mode

Explains the remote switch for controlling laser mode with external lasers.

Connecting the Remote Switch for Laser Mode

Guide for connecting the remote switch for laser mode.

Basic kit laser safety Rev. 2

Information on the basic laser safety kit for external lasers.

Connecting the Slider Laser TIRF mot.

Steps for connecting the motorized Slider Laser TIRF.

Coupling a Laser to the Laser TIRF Imaging System

Procedure for coupling an external laser to the system.

Safety Check Prior to First Start-up

Checklist for ensuring safety before initial system startup.

Power Requirements

Details power requirements for systems with customer lasers.

APPENDIX

List of Abbreviations

Provides a list of abbreviations used in the manual.

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