Zeiss Axio Imager A1 User Manual

Cancer Institute Microscopy Core Facility
V1.0
ZEISS AXIO IMAGER A1 AND M1
A USERS GUIDE
This is intended to provide a basic coverage of use of the Zeiss Axio Imager M1 for multi-
channelled fluorescent acquisition. For more complex usages please contact
Fig.1 – Front View of Microscope
Ocular Viewer
Main Stage
Mercury Lamp
(Reflected light)
Control Box
Microscope Power
Supply Box
Microscope
control
touch-screen
Viewing
Slide
1
st
Focusing
Wheel

Zeiss Axio Imager A1 Specifications

General

Power Supply	100-240V AC, 50/60Hz
Microscope Type	Research Microscope
Optical System	Infinity Optical System
Objectives	Plan-Apochromat, Plan-Neofluar
Eyepieces	10x
Illumination	LED or Halogen
Stage	Mechanical stage
Focusing	Coarse and fine focusing
Camera Ports	C-mount
Contrast Methods	Brightfield, Darkfield, Phase contrast, Fluorescence
Software	ZEN software

Summary

Switching On

Locate Focal Plane of Sample

Acquisition of Images

Open the Z tab

Instructions for opening the Z tab in the acquisition software interface.

Image Processing and Annotation

Viewing Your Image or Z-Stack

Guidance on how to view acquired images or Z-stacks within the software.

Deconvolution of Z-Stack

Explanation of the deconvolution process to clean up images using Axiovision.

Orthoview

Using the Orthoview option to average and overlay Z-Stack images into a 2D view.

Annotations

Adding annotations like text, arrows, and scale bars to images for publication.