Zeiss Axio Imager M1 User Manual

Cancer Institute Microscopy Core Facility
V1.0
ZEISS AXIO IMAGER A1 AND M1
A USERS GUIDE
This is intended to provide a basic coverage of use of the Zeiss Axio Imager M1 for multi-
channelled fluorescent acquisition. For more complex usages please contact
Fig.1 – Front View of Microscope
Ocular Viewer
Main Stage
Mercury Lamp
(Reflected light)
Control Box
Microscope Power
Supply Box
Microscope
control
touch-screen
Viewing
Slide
1
st
Focusing
Wheel

Zeiss Axio Imager M1 Specifications

General

Microscope Type	Upright Microscope
Optical System	Infinity Color-Corrected System (ICS)
Light Source	Halogen, LED
Stage Type	Motorized or manual stage options
Software	ZEN (Zeiss Efficient Navigation)
Objectives	Plan-Apochromat, Plan-Neofluar
Illumination	Transmitted and reflected light
Contrast Methods	Brightfield, Darkfield, Phase Contrast, DIC, Fluorescence
Focusing	Coaxial coarse and fine focusing
Eyepieces	10x
Nosepiece	Motorized or manual
Camera Compatibility	Various digital cameras via adapter
Camera Port	Integrated camera port with C-mount adapter

Summary

Switching On the Microscope

Locate Focal Plane of Sample

Acquisition of Images

Axiovision Tool Bar and Workarea

Navigating the Axiovision software interface and selecting the workarea and objective for image capture.

Image Processing and Annotation

Z-stack Viewing and Channel Management

Methods for viewing captured Z-stacks, managing channels, and scrolling through images using the software.

Deconvolution of Z-Stack

Utilizing Axiovision's deconvolution feature to improve image quality by removing aberrations and chromatic shifts.

Orthoview Projection

Image Annotations

Scale Bar Setup

Procedure for setting image scale and drawing scale bars using the scaling tab feature in the software.