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General| Brand | Zeiss |
|---|---|
| Model | ELYRA |
| Category | Laboratory Equipment |
| Language | English |
Recommends high-precision coverslips (no. 1.5 thickness) for mounting samples.
Discusses suitable organic dyes and fluorescent proteins for SR-SIM, emphasizing specific labeling.
Lists embedding media with specific refractive indices (RI) for sample preparation.
Recommends Nunc Lab-Tek chambers or glass bottom dishes for PALM/dSTORM sample preparation.
Explains the advantage of photoswitchable fluorescent proteins (PS-FPs) for PALM and lists examples.
Lists recommended fluorescent protein pairs for dual-color PALM imaging experiments.
Discusses suitable organic dyes for dSTORM, focusing on photon yield, duty cycle, and survival fraction.
Suggests direct antibody labeling or using nanobodies for dSTORM due to antibody size.
Describes post-fixation as a step to improve label stability and prevent detachment.
Provides a recipe and notes for preparing the imaging buffer for dSTORM experiments.
Explains the purpose of fiducial markers for drift correction, channel alignment, and autofocus.
Lists suppliers and catalog numbers for fiducial markers and coverslips.
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