Zeiss ELYRA - Embedding for Superresolution Microscopy; Embedding Media

Embedding
Ideally, the sample should be embedded in a medium that matches the refractive index (RI) of the immersion oil
(n=1,518).Thefollowingmediaperformwell,despitehavingalowerrefractiveindex.
• Fluoromout-G(SouthernBiotech)withRIof1,40.
• 86%Glycerolwith2.5g/lDABCO(1,4-deazabicyclo[2.2.2]octane,SigmaCat.D2522)inTris-HCl.1MpH8.0.
 Forfurtherreading:http://www.nanoimaging.de/ homepages  Sample requirements
• Non-hardeningVECTASHIELDMountingMediumwithRIof1,44.
• ProLongGold(LifeTechnologies).Beawarethisembeddingmediumneeds2daytoharden(inordertoreacha
constant RI). In addition your sample may shrink during this process. Refractive index (RI) of the cured product:
1,46.
• SlowFadeGold(LifeTechnologies)withRIof1,42.
• 2,2’-thiodiethanol(TDE)–aqueous.RIcanbevariedrangingfrombeingthatofwater(1.33)tothatofimmersion
oil(1.52)byappropriatelydilutingwithwater.
Note
In order to have stable imaging conditions, especially concerning the RI of the mounting medium we would urge
you to prepare the slides at least one week before use. Please note, that during curing the RI will rise.
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