Zeiss ELYRA - Fluorescent Labels for SR-SIM; Labeling for SR-SIM

Please make sure that the coverslip is centered on the glass slide in order to fit into the sample holder.
SeebelowpicturesoftheZEISSleveladjustablepiezostageinsert.
Fix your cells according to your standard protocol. For SR-SIM imaging, a thoroughly clean glass surface plays a crucial
role.Thereforeitisbenecialtosealorattachthecoverslipinawaythatfacilitatescleaningwithethanol,without
moving the coverslip.
Fluorescent Labels for SR-SIM
Allcommontypesoforganicdyesusuallyconjugatedtoantibodiesoruorescentproteinsaresuitabletobeusedfor
SR-SIM. Make sure to have a highly specific labeling with low background to obtain a good signal to noise ratio.
Formulticolorsamplestheuorophoresshouldbeselectedforminimalspectraloverlaptoavoidcrosstalk.Available
ltersetsforELYRAPS.1areareoptimizedfortheavailablelaserlines:405,488,561and642nm.
Note
Cytosolicorothernon-specicuorescentproteinexpression(e.g.GFP)willresultinstainingofextendedareas.
Since well-defined structures are missing to interfere with the grid pattern, modulation contrast will be low and the
final image will lack high resolution information.
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Figure 1 ZEISS level adjustable piezo stage insert