Zeiss ELYRA - General Guidelines for Superresolution Structured Illumination Microscopy (SR-SIM); Sample support for SR-SIM

General Guidelines for
Superresolution Structured Illumination Microscopy (SR-SIM)
Sample support
In general, we recommend using high-precision coverslips (always no. 1.5 thickness) to mount the samples.
High-precision cover glasses feature an exceptionally accurate thickness of 170 ± 5 µm (= 0,170 ± 0,005 mm).
Also mind thickness issues when ordering glass-bottom petri dishes or multi-well plates.
For cover glasses see:
• ZEISSHighprecisionCoverGlasses18x18mm;
ZEISSorderNumber:474030-9010-000
• MarienfeldSuperior:www.marienfeld-superior.com
 •18x18mm(Cat.No.0107032).
 •22x22mm(Cat.No.0107052).
 •roundwith18mmdiameter(Cat.No.0117580).
• CellPathLtdUKHighperformancecoverslips
no1.5H18x18mm(Cat.NoSAN-5018-03A)
For glass-bottom petri dishes and multi-well plates see:
• Willco(http://www.willcowells.com/)
• Lab-tek(offeredthroughThermo)
• MatTek(http://glass-bottom-dishes.com)
ZEISS ELYRA
Sample Preparation for Superresolution Microscopy –
a Quick Guide
Authors: Dr. Sylvia Münter
  Dr.YilmazNiyaz
  CarlZeissMicroscopyGmbH,Germany
Date:  November2013
White Paper
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