Zeiss ELYRA - General Guidelines for Photoactivated Localization Microscopy (PALM); Sample support for PALM

General Guidelines for Photoactivated Localization Microscopy (PALM)
Sample support
SamplesforPALManddSTORMareideallypreparedon
NuncLab-Tekchambersorglassbottomdisheswithcover
glassthicknessNo1.5.
• NuncLab-TekIIChamberedCoverglassno1.5
(orderno.:155409)
• GreinerBio-One(orderno.:672860CELLview)
• NuncLab-Tekchambers(orderno.:
 P35G-0.17-10-CorP35G-0.17-14-C)
Alltheseformatswilltintotheadjustablepiezostage
insert.
Pleasebeaware,inordertoobtainidealTIRFillumination
you need a sufficient mismatch in refractive indices.
In addition you may want to change imaging buffer
concentration therefore we recommend no embedding
afterxationofPALManddSTORMsamples.
Note
NuncLab-Tekchambersarewellsuitedtotransportsamplesbetweenlabs.Theytperfectlyintoa50mlFalcontube,
thatcanbelledwithPBSandsealedwithparalm.
White Paper
5
Figure 2 ZEISS level adjustable piezo stage insert