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(English)
EN
Issue Date: 2015-08
Product Instructions
Select E. coli Count Plate
Product Description And Intended Use
The 3M™ Petrilm™ Select E. coli Count (SEC) Plate is a sample-ready-culture medium system which contains
selective agents, nutrients, a cold-water-soluble gelling agent, and an indicator of glucuronidase activity, 5-bromo-
4-chloro-3-indolyl-D-glucuronide (BCIG), which facilitates colony enumeration. 3M Petrilm SEC Plates are used for
the enumeration of Escherichia coli (E. coli) in the food and beverage industries. Most E. coli strains are thermotolerant
and produce beta-glucuronidase, an enzyme that reacts with BCIG indicator in 3M Petrilm SEC Plates to produce
dark green to blue-green colonies. 3M Petrilm SEC Plate components are decontaminated though not sterilized. 3M
Food Safety is certied to International Organization for Standardization (ISO) 9001 for design and manufacturing. 3M
Petrilm SEC Plates have not been tested with all possible food products, food processes, testing protocols or with all
possible strains of E. coli or other bacteria.
Safety
The user should read, understand, and follow all safety information in the instructions for the 3M Petrilm SEC Plate.
Retain the safety instructions for future reference.
WARNING: Indicates a hazardous situation, which, if not avoided, could result in death or serious injury and/or
property damage.
CAUTION: Indicates a hazardous situation, which, if not avoided, could result in minor or moderate injury and/or
property damage.
W WARNING
E. coliE. coli
atypical E. coli
• Follow all product storage instruction contained in the instructions for use.
• Do not use beyond expiration date.
• Perform 3M Petrilm SEC Plate testing in a properly equipped laboratory under the control of a skilled
microbiologist.
• The user must train its personnel in current proper testing techniques: for example, Good Laboratory Practices
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, ISO
17025
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, or ISO 7218
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.
• 3M has not documented 3M Petrilm SEC Plates for use in industries other than food and beverage. For example,
3M has not documented 3M Petrilm SEC Plates for testing water, pharmaceuticals, or cosmetics.
• Do not use 3M Petrilm SEC Plates in the diagnosis of conditions in humans or animals.
• 3M Petrilm SEC Plates do not dierentiate any one E. coli strain from another.
• High sugar content foods may decrease the color reaction of the glucuronidase and produce pale green to colorless
colonies.
• Follow current industry standards and local regulations for disposal of contaminated waste.
CAUTION
A few strains of bacteria other than E. coli, such as some Shigella, Salmonella, Enterobacter and Klebsiella, can produce
beta-glucuronidase and will produce dark green to blue-green colonies on the 3M Petrilm SEC Plate, especially when
incubated at lower than recommended temperatures
4
.
Consult the Safety Data Sheet for additional information.
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