3M Petrifilm - Specific Instructions for Validated Methods; References and Symbols

4
(English)
EN
󰄈
󰄈
NF Validation certied method in compliance with ISO 16140
7
in comparison to ISO 16649-2
8
.
Use the following details when implementing the above Instructions for Use:

For testing all human food products, pet food and industrial environmental samples.

Use only ISO listed diluents.

Incubate 3M Petrilm SEC Plates 24 hours ± 2 hours at 42ºC ± 1ºC.
Interpretation:
Calculate the number of microorganisms present in the test sample according to ISO 7218
3
for one plate per dilution. For
calculation, take into account only 3M Petrilm SEC Plates that contain up to 150 colonies. Estimates are outside of the
scope of the NF Validation Certication (cf. interpretation part paragraph 3).
3M 01/08 –06/01

http://nf-validation.afnor.org/en
For more information about end of validity, please refer to NF VALIDATION certicate available on the website
mentioned above.
References
1. U.S. Food and Drug Administration. Code of Federal Regulations, Title 21, Part 58. Good Laboratory Practice for
Nonclinical Laboratory Studies.
2. ISO/IEC 17025. General requirements for the competence of testing and calibration laboratories.
3. ISO 7218. Microbiology of food and animal feeding stus – General rules for microbiological examination.
4. Frampton, E.W. and L. Restaino, 1993. Methods for Escherichia coli identication in food, water and clinical samples
based on beta-glucuronidase detection. Journal of Applied Bacteriology 74, 223-233.
5. FDA. Bacteriological Analytical Manual (BAM), 8th Edition, Revision A, 1998. Reagents Index for BAM found at:
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm055791.htm.
6. ISO 6887-1 Microbiology of food and animal feeding stus – Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination.
7. ISO 16140. Microbiology of food and animals feeding stus – Protocol for the validation of alternative methods.
8. ISO 16649-2: Microbiology of food and animal feeding stus- Horizontal method for the enumeration of
ß-glucuronidase-positive Escherichia coli - Part 2: Colony- count technique at 44°C using 5-bromo-4-chloro-3-
indolyl ßD-glucuronide.
Refer to the current versions of the standard methods listed above.

www.3M.com/foodsafety/symbols
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