Getting Started
Step 3. Prepare the Triple Quad instrument
Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide 17
3 Try the following quick changes to get Checktune to pass.
a Click the Manual Tune tab.
b If the failure occurred in MS1, click the MS1 tab. Select the Peak Width
based on which resolution failed in Checktune. You can select Unit, Wide,
or Widest. Then, click the Adjust Gain & Offset button.
c If the failure occurred in MS2, click the MS2 tab. Select the Peak Width
based on which resolution failed in Checktune. Then, click the Adjust Gain
& Offset button.
d If the Adjust Gain and Offset passes successfully, then save the autotune
file. Click Manual Tune. Then, click the Files tab, and click Save.
e Click the Autotune tab.
f Click the Checktune button.
If Checktune fails again, you must run an Autotune, which is described next.
4 Click Autotune to tune the MS automatically (approximately 30 minutes for
each ionization mode for all models except the 6490 and the 6495. The 6490 and
6495 take approximately 25 minutes per ionization mode.). The system
automatically changes different tune parameters to tune the MS. You only do an
Autotune when it is necessary.
For all instruments except the 6490 and the 6495, you can run an Autotune
with the following sources: AJS ESI, MMI, and HPLC-Chip. With the 6490 and
6495, you can only run an Autotune with the AJS ESI source.
If Autotune fails, then you mark the Start from Factory Defaults check box.
Then, if you click Autotune, the instrument is tuned starting from the factory
defaults (approximately 30 minutes for each polarity for all models except the
6490 and the 6495. The 6490 and 6495 take approximately 25 minutes per
ionization mode.).
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