Other Operating Tips
(continued)
• Columns should not be maintained at elevated pH or elevated
temperature when not in use (see Table 2).
• New columns may contain a mixture of organic solvents and water,
which may contain buffer salts. See Table 3 for details of the shipping
solvents. Initially, care should be taken not to pass any mobile phase
through the column that may cause a precipitate to form or may not
be fully miscible.
• Agilent BioHPLC and AdvanceBio columns are compatible with all
common eluents used for analysis of biomolecules.
ENGLISH
Column Particle size Pressure limit
AdvanceBio Peptide Mapping
2.7 µm
600 bar
AdvanceBio Peptide Plus
AdvanceBio Oligonucleotide
AdvanceBio Amino Acid Analysis
AdvanceBio RP-mAb 3.5 µm
ZORBAX 300SB 3.5 µm, 5 µm, 7 µm
400 bar
Poroshell 300 5 µm
ZORBAX RRHD 1.8 µm 1200 bar
PLRP-S
3 µm 275 bar
5 µm, 8 µm, 10 µm 207 bar
10-15 µm, 15-20 µm, 30 µm 103 bar
Table 1. Maximum operating parameters – columns
up to 4.6 mm
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