Agilent Technologies BioHPLC series User Manual

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Column
Recommended
pH range
Maximum operating
temperature
AdvanceBio Peptide Mapping
2.0-8.0 60 ºC
AdvanceBio Peptide Plus
ZORBAX RRHD
1.0-8.0 80 ºC
ZORBAX 300SB-C8, C3, CN
ZORBAX 300SB-C18
1.0-8.0 90 ºCAdvanceBio RP-mAb
Poroshell 300
Poroshell 300 Extend-C18 2.0-11.0 60 °C below pH 8,
40 °C above pH 8
AdvanceBio Oligonucleotide
3.0-11.0 65 ºC
AdvanceBio Amino Acid Analysis
PLRP-S 1.0-14.0 200 ºC
Table 2. Column operating parameters – pH and temperature
Note: All silica-based packings have some solubility in pH >6 aqueous mobile phases. When using silica-
based columns at pH >6, best column lifetime is obtained at lower temperatures (40 °C max) using low buffer
concentrations in the range of 0.01 to 0.02 M.
Column Shipping solvent Compatibility
AdvanceBio Peptide Mapping
Mixture of acetonitrile
and water
Water and all common organic
solvents.
AdvanceBio Peptide Plus
ZORBAX RRHD
ZORBAX 300SB
AdvanceBio RP-mAb Mixture of acetonitrile
and water
Water and all organic solvents,
including N,N-dimethylformamide
and dimethylsulfoxide.
Poroshell 300 and 300 Extend Mixture of methanol
and water
AdvanceBio Oligonucleotide
Mixture of acetonitrile
and water
Water and all common organic solvents.
Modifiers including HFIP and TEAA.
AdvanceBio Amino Acid Analysis
PLRP-S 7:1 acetonitrile/water Aqueous organic solvents
including N,N-dimethylformide and
dimethylsulfoxide. 100% aqueous is
not recommended as it will reduce
column performance and lifetime.
Table 3. Shipping solvents

Agilent Technologies BioHPLC series Specifications

General

Brand	Agilent Technologies
Model	BioHPLC series
Category	Laboratory Equipment
Language	English