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150 Series

Operation

Setting Up

1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly

in place with the metal slide holders (clips) of the mechanical stage.

2. Center the specimen over the stage opening, lining it up with the light and the objective lens.

3. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of

light is achieved.

Focusing

1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnication rst (4x objective) to lo-

cate and focus on the specimen. As you move up in magnication you may need to refocus the image a little each

time.

2. Being focusing by rst looking with one eye through the eyepiece without the diopter. Close your other eye. Use

the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.

Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the full range

of motion for ne tuning the focus.

3. Once the image is clear in your eld of view, you will want to use the ne focusing knob to tune it for best results.

Note: Please be careful when moving the mechanical stage if you need to recenter the sample, or if moving the

stage very close to the objectives. e limit stop is designed to prevent impact between objective and slide, so when

it is o you will be able to damage the microscope.

Adjusting The Diaphragm

1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the

aperture of the iris diaphragm by rotating the disc underneath the stage to the desired aperture.

Attaching a Camera / Changing Eyepieces

1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal screw on the

ocular tube just under where the eyepiece connects to the tube. To do so, you will need a 1mm athead precision

screwdriver (much like what you would use to adjust eye glasses).

AmScope 150 Series User Manual

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