PRINCIPLES OF OPERATION
•
101
Chapter 8
3) The correspondence between the command potential (V
CMD
) and the transmembrane
potential (V
m
) is:
Cell-attached patch V
m
= RMP - V
c
Inside-out patch V
m
= - V
c
Outside-out patch V
m
= V
c
Whole-cell voltage clamp V
m
= V
c
Troubleshooting
It has been our experience at Axon Instruments that the majority of troubles reported to us
have been caused by faulty equipment connected to our instruments.
If you have a problem, please disconnect all instruments connected to the Axopatch 200B
except for the headstage. Ideally, remove the Axopatch 200B from the rack. Work
completely through the Functional Checkout. This can often uncover a problem that is in
your set up. If the problem persists, please call us for assistance.
Another common problem is caused when dirt or corrosion build up in the headstage
connector socket. This can cause unstable current and voltage offsets. It is important to
keep the holders and the headstage input clean.
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