Axon Axopatch 200B User Manual

TABLE OF CONTENTS
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iii
Axopatch 200B, Copyright 1997-1999, Axon Instruments, Inc.
TABLE OF CONTENTS
Page
LIST OF FIGURES AND TABLES ................................................................................... vii
CHAPTER 1: INTRODUCTION..........................................................................................1
CHAPTER 2: FUNCTIONAL CHECKOUT........................................................................3
CHAPTER 3: USE OF THE PATCH CLAMP - A TUTORIAL........................................11
Single Channel Recording (Model Cell) ......................................................................12
Pipette Offset Adjustment ............................................................................................12
Using the TRACK Mode ......................................................................................12
Using the V-CLAMP Mode..................................................................................13
Adjustment of Pipette Capacitance Compensation ......................................................14
Adjustment of Leak Subtraction...................................................................................14
Whole-Cell Recording (Model Cell) ............................................................................15
Pipette Offset Adjustment ............................................................................................16
Whole-Cell Capacitance Compensation.......................................................................16
Series Resistance Compensation ..................................................................................19
Brief Method For Setting Series Resistance Compensation.................................19
Detailed Method For Setting Series Resistance Compensation ...........................22
Current Clamp (Model Cell).........................................................................................28
Single-Channel Recording (Real Cell) .........................................................................30
Whole-Cell Recording (Real Cell) ...............................................................................35
CHAPTER 4: INTERFACING A COMPUTER TO THE AXOPATCH 200B .................41

Axon Axopatch 200B Specifications

General

Brand	Axon
Model	Axopatch 200B
Category	Industrial Equipment
Language	English