Axon Axopatch 200B - Whole-Cell Recording (Model Cell)

USE OF THE PATCH CLAMP – A TUTORIAL
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15
Chapter 3
CONDITIONS:
PATCH CONFIG.
SEAL TEST ON 5kHz FILTER
Figure 2.
Electrode (pipette) capacitance compensation.
Whole-Cell Recording (Model Cell)
Set the front panel controls of the Axopatch 200B as follows:
PIPETTE OFFSET: About 5.0
ZAP: 0.5 ms
SERIES RESISTANCE COMP. % PREDICTION: 0 %, OFF
SERIES RESISTANCE COMP. % CORRECTION: 0 %, OFF
SERIES RESISTANCE COMP. LAG: 1 µs
WHOLE CELL CAP.: 0 pF, OFF
SERIES RESISTANCE: 0 MΩ
HOLDING COMMAND: 0 mV, toggle x1, OFF
SEAL TEST: OFF
METER: Set switch to V
TRACK
MODE: TRACK
CONFIG.: WHOLE CELL (β = 1)
OUTPUT GAIN: α = 1
LOWPASS BESSEL FILTER: 5 kHz
LEAK SUBTRACTION: ∞ MΩ, OFF
↑↑
PULSE PULSE
ON OFF
1
11
1 p
pp
pA
AA
A
1
11
1 m
mm
ms
ss
s