INSTRUMENT OPERATION
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Chapter 6
Frequency Boosting
The WHOLE CELL mode of the Axopatch 200B uses a resistor in the headstage feedback
that requires frequency boosting (tuning) for optimal performance. The frequency boosting
circuit is tuned at the factory and will usually not require readjustment in the field. If
tuning is required, see the
HEADSTAGE
TUNING PROCEDURE
(page 121).
Case Ground Connector
The metal headstage case and the gold-plated 1 mm socket at the rear of the headstage are
connected to ground. Use this ground for grounding the preparation.
No provision is made for driving a shield since using a driven shield around the pipette
increases the high-frequency noise.
Headstage Dimensions
The headstage box measures 0.7" wide x 0.75" high x 4.2" long.
Mounting the Headstage
The improved, slim design of the CV 203BU headstage makes it easier to guide your
pipette towards your preparation without physical interference from other instruments in
your set-up. For maximum mechanical rigidity, the headstage can be mounted directly to
some manipulators using the mounting plate located on the bottom of the headstage. In
addition, the bottom of the headstage has been engineered for stable attachment to user-
designed mounts.
Cleaning
Wipe the headstage connector with a damp cloth to clean salt spills. Avoid spilling liquids
on the headstage.
The Teflon input connector should be kept very clean. Effective cleaning can be done by
spraying with alcohol or swabbing carefully with deionized water. If possible, avoid the
use of Freon since it is thought to be detrimental to the environment.
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