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FUNCTIONAL CHECKOUT
Axopatch 200B, Copyright 1997-1999, Axon Instruments, Inc.
2) Set the front panel controls of the Axopatch 200B as follows:
PIPETTE OFFSET: About 5.0
ZAP 0.5 ms
PIPETTE CAPACITANCE COMP.: Minimum
(fully counterclockwise)
SERIES RESISTANCE COMP. % PREDICTION: 0 %, OFF
SERIES RESISTANCE COMP. % CORRECTION: 0 %, OFF
SERIES RESISTANCE COMP. LAG: 1 µs
WHOLE CELL CAP.: 0 pF, OFF
SERIES RESISTANCE: 0 MΩ
HOLDING COMMAND: 0 mV, x1, OFF
SEAL TEST: OFF
METER: Set switch to I
MODE: V-CLAMP
CONFIG.: WHOLE CELL (β = 1)
OUTPUT GAIN: α = 10
LOWPASS BESSEL FILTER: 5 kHz
LEAK SUBTRACTION: ∞ MΩ, OFF
Test the noise:
3) Shield the headstage with a large sheet of aluminum foil. Wrap the foil loosely and
completely around the headstage. Leave room at the headstage input for the model cell
in the next test. Connect the foil shield to the ground input of the headstage (gold-
plated 1 mm socket at the rear of the probe) using a clip-lead. The easiest way to do
this is to connect the clip-lead directly to the foil and to the 1 mm pin inserted into the
headstage ground socket. (Ground is available from the gold-plated 1 mm socket at the
rear of the probe, from the yellow 4 mm socket on the rear-panel of the main unit, or
from the BNC shields.)
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