BD Phoenix M50 System - Page 9

BD Phoenix™ M50 System Quick Reference Guide
Preparing BD Phoenix™ EMERGE™ Panels
Option 1: Two tubes of AST broth will be required.
1 Follow Steps 1–10 (refer to Section 6.2.3 General Panel Preparation of the BD Phoenix™ M50 System User’s Manual) to prepare the suspension
of bacteria.
2 Label two BD Phoenix™ AST Broth tubes (8.0 mL) with the patient's specimen number. Add one drop of the BD Phoenix™ AST Indicator to each
AST broth tube. Invert to mix. DO NOT VORTEX.
3 Transfer 25 μL (50 μL if low inoculum option is used) of the suspension to two BD Phoenix™ AST Broth tubes. Cap and gently invert.
4 Using sterile technique, remove 3.5 mL of broth from one of the inoculated BD Phoenix™ AST Broth tubes and discard in an appropriate container.
5 Pour the remaining 4.5 mL into the left side of the BD Phoenix™ EMERGE™ panel. Allow the fluid to traverse down the tracks before moving the
panel.
6 Pour the other BD Phoenix™ AST Broth tube into the right side of the BD Phoenix™ EMERGE™ panel. Allow the fluid to traverse down the tracks
before moving the panel.
7 Snap on the panel closures and follow the normal panel login procedure.
Option 2: BD Phoenix™ EMERGE™ AST Broth (12.5 mL) and BD Phoenix™ EMERGE™ AST Indicator are required.
1 Follow Steps 1–10 (refer to Section 6.2.3 General Panel Preparation of the BD Phoenix™ M50 System User’s Manual) to prepare the suspension
of bacteria.
2 Label a BD Phoenix™ EMERGE™ AST Broth tube (12.5 mL) with the patient's specimen number. Add one drop of BD Phoenix™ EMERGE™
AST indicator into the Emerge AST Broth, cap and gently invert to mix. DO NOT VORTEX.
3 Transfer 39 μL (78 μL if low inoculum option is used) of the suspension to the BD Phoenix™ Emerge AST broth with indicator. Cap and invert
several times to mix. DO NOT VORTEX.
4 Pipette 4.5 mL of inoculated AST broth into the 51-well side (left side) of the BD Phoenix™ Emerge panel. Allow the fluid to traverse down the
tracks before moving the panel.
5 Pour the remaining inoculated Emerge AST broth into the 85-well side (right side) of the BD Phoenix™ Emerge panel. Allow the fluid to traverse
down the tracks before moving the panel.
6 Snap on the panel closures and follow the normal panel login procedure.
9
BD EMERGE™ Panel Prep