BIO RAD 680 - Page 57

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1-3. Absorbance Data
1-3-1. Abs data
<abs.data>: <header><date time><reading info><Mes. data><cr>[<Ref. wavelength data><cr> ]
Ref.data present for dual wavelength readings.
<header>: “BIO-RAD Model 680 Microplate READER”<cr>
<date time>: <day> ”/” <month> “/” <year> <space> <hour> ”:” <minutes> “:” <seconds> ><cr>
ex. “01/01/2001 01:05:02”
<reading info>: “Mes. filter:”<measurement filter wavelength data><cr>
“Ref. filter:”<reference filter wavelength data><cr>
Only when Dual wavelength reading
<Mes.data>: “ begin”<cr><8 rows of 12 wells of abs. values><checksum><cr>”.end”<cr>
Measurement wavelength absorbance values are in ASCII encoded floating point
format. Each absorbance value is separated by a space, but in case of minus value, a
minus sign is put instead.
Row1=wells(A1-A12),Row2=wells(B1-B12),….Row8=wells(H1-H12)
<Ref.data>: “.begin”<cr><8 rows of 12 wells of abs. values><checksum><cr>”.end”<cr>
Reference wavelength absorbance values are in ASCII encoded floating point format.
Each absorbance value is separated by a space, but in case of minus value, a minus
sign is put instead.
Row1=wells(A1-A12),Row2=wells(B1-B12),….Row8=wells(H1-H12)
<checksum>: The checksum is a two byte sum in ASCII encoded decimal(0-255) of all the ASCII
characters transmitted in the measurement or reference data block. The checksum
does not include the character string “.begin”<cr> in the checksum calculations.
The following is an example of both <Mes.data> and <Ref.data>:
“.begin”<cr>
“ 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 *.***”<cr>
“ 0.201 0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 *.***”<cr>
“ 0.301 0.302 0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 *.***”<cr>
“ 0.401 0.402 0.403 0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 *.***”<cr>
“ 0.501 0.502 0.503 0.504 0.505 0.506 0.507 0.508 0.509 0.510 0.511 *.***”<cr>
“ 0.601 0.602 0.603 0.604 0.605 0.606 0.607 0.608 0.609 0.610 0.611 *.***”<cr>
“ 0.701 0.702 0.703 0.704 0.705 0.706 0.707 0.708 0.709 0.710 0.711 *.***”<cr>
“ 0.801 0.802 0.803 0.804 0.805 0.806 0.807 0.808 0.809 0.810 0.811 *.***”<cr>
<checksum><cr>
“.end”<cr>
Note: Absorbance value greater than 3.500 or smaller than –3.500 shall be transmitted as asterisk.