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Biotage Isolera One - User Manual

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Isolera
User Manual

Table of Contents

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Summary

Description and Specifications

General System Description

Provides an overview of the Isolera system's basic components and configurations.

Basic System Components

Details the core components of the Isolera system, including display, collector, and pump.

System Configurations

Compares the four available Isolera system platforms: Prime, One, Four, and LS.

Programmed Functions

Lists the automated functions the Isolera system can perform for purification processes.

Software Description

Explains the different software modes (Chemistry, Data Administration, System) and their features.

Data Administration Mode

Details the functions available within the Data Administration mode, organized by tabs.

System Mode

Outlines the settings and maintenance tasks controllable via the System mode.

Chemistry Mode

Explains the Chemistry mode interface and functionalities for setting up and running purifications.

Method Tab (Chemistry Mode)

Covers creating, opening, and editing purification methods within the Chemistry mode.

Status Tab (Chemistry Mode)

Describes how to view and monitor a purification's progress in the Status tab.

Results Tab (Chemistry Mode)

Explains how to search, view, and manage purification results and reports.

Specifications

Lists the technical specifications for the Isolera system.

General System Specifications

Details power, certification, dimensions, environmental, and interface specifications.

Operational Performance Specifications

Outlines performance specifications for cartridge positions, solvents, fraction collector, and detectors.

Data Administration

Log into the Data Administration Mode

Step-by-step guide on how to access the Data Administration mode.

Administrate the Cartridge List

Instructions for managing cartridge types, adding, editing, or deleting them.

Administrate the Method List

Covers managing purification methods: export, import, delete, and set defaults.

Administrate the Rack List

Process for managing collection rack types, including adding and editing.

Administrate the Result List

Covers exporting, deleting, and cleaning up result records from the database.

Administrate the Solvent List

Instructions for managing solvent lists, including adding, editing, or deleting.

Administrate the User List

Covers managing user accounts: adding, editing, and deleting.

System Settings

Log into the System Mode

Step-by-step guide on how to access the System mode.

Change Detector Settings

Instructions for configuring internal, external, and leak detectors.

Connect and Enable an External Detector

Guide on connecting and enabling an external detector, like ELSD-1080.

Configure a Network Connection

Steps for setting up network connectivity for reports and remote access.

Change Report Settings

Configuration options for printing, saving, and sending reports.

Change Runtime Settings

Adjustments for system behavior during operation, like alarms and allocation.

Maintenance

Procedures for system upkeep, calibration, and troubleshooting.

Operation (Chemistry Mode)

Start Up the System

Step-by-step instructions for safely starting up the Isolera system.

Shut Down the System

Procedure for performing an orderly system shutdown to prevent data corruption.

Create, Open, and Edit Methods

Overview of creating, opening, and modifying purification methods.

Create or Open a Method

Guides on creating new methods or opening existing ones for purification setup.

Prepare and Run a Purification

Steps to prepare the system and execute a purification run.

Prepare the System for a Run

Actions required before starting a purification, like loading samples and cartridges.

Run a Purification

Detailed steps for initiating and managing a purification run.

Monitor and Control a Purification

How to monitor purification progress and control various aspects during a run.

Monitor the Purification in Progress

Using the Status tab to view gradient, chromatogram, and spectrum data.

Pause, End, or Abort a Purification

Procedures for pausing, ending, or aborting an ongoing purification.

Unload a Purification

Steps for unloading cartridges and racks after a purification is finished.

Access Result Records

How to search, view, and manage stored purification results.

View, Print, and Save Reports on a USB Memory Device or the Network

How to view, print, and save purification reports to external media or network.

Solvent and Waste Handling

Procedures for managing solvent and waste reservoirs.

Prime the System

Steps to prime solvent inlets and the system for purification.

Maintenance

Leaks

Safety precautions and procedures for handling system leaks.

Shut Down the System

Steps to shut down the system if a leak is detected.

Troubleshooting

Fraction Collector-Related Problems

Troubleshooting guide for issues related to the fraction collector.

Pump-Related Problems

Troubleshooting guide for issues related to the system's pump.

Internal Detector-Related Problems

Troubleshooting guide for issues with the internal detector.

Gradient Problems

Troubleshooting guide for issues encountered with gradient formation.

Leak Detected

Reference to troubleshooting steps for leaks.

Overpressure Detected

Troubleshooting guide for overpressure conditions in the system.

Contact Information

Technical Support

Provides contact information and required details for technical support.

Biotage Isolera One Specifications

General IconGeneral
Detection MethodUV/VIS
Number of Solvents4
DisplayTouchscreen
Maximum Pressure20 MPa (200 bar)
Gradient ProfilesLinear, step, or custom
Fraction CollectionYes
Sample LoadingManual or automated
Power Requirements100-240 V, 50/60 Hz

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