411829-L, Operation April 2012 Page 4-21
4
* Cannot be used on Isolera LS.
†
When using Isolera Prime, the maximum flowrate is limited to 100 ml/min.
When using Isolera One or Isolera Four, the maximum flow rate is limited to 200 ml/min.
‡
Not recommended for use on Isolera Prime, Isolera One, or Isolera Four.
§
Not recommended for use on Isolera Prime.
Table 4-3: Load Capacity Under Gradient Conditions
Cartridge
Column
Volume
(ml)
Silica
Weight
(g)
Load Capacity
CV 0.1-1.9
(mg)
Load Capacity
CV 2.0-3.9
(mg)
Load Capacity
CV 4.0+
(mg)
Flow Rate
(ml/min)
SNAP 10g* 15 10 100-200 200-350 350-500 10-20
SNAP 25g* 33 25 250-500 500-875 875-1250 20-40
SNAP 50g 66 50 500-1000 1000-1750 1750-2500 50-100
SNAP 100g 132 100 1000-2000 2000-3500 3500-5000 50-100
SNAP 340g 510 340 3400-6800 6800-8900 8900-17000
65-300
†
SNAP 750g
§
990 750 7500-15000 15000-26000 26000-35500
100-500
†
SNAP 1500g
‡
1980 1500 15000-30000 30000-52000 52000-76000 300-500
SNAP HP 10g* 15 10 200-400 400-700 700-1000 10-20
SNAP HP 25g* 33 25 500-1000 1000-1750 1750-2500 20-40
SNAP HP 50g 66 50 1000-2000 2000-3500 3500-5000 50-100
SNAP HP 100g 132 100 2000-4000 4000-7000 7000-10000 50-100
SNAP HP 340g 510 340 6800-13600 13600-17800 17800-34000
65-300
†
SNAP Ultra 10g* 17 10 <200 200-1000 1000-2000 10-50
SNAP Ultra 25g* 45 25 <500 500-2500 2500-5000 20-100
SNAP Ultra 50g 85 50 <1000 1000-5000 5000-10000
30-150
†
SNAP Ultra 100g 164 100 <2000 2000-10000 10000-20000
30-150
†
SNAP Ultra 340g 582 340 <6800 6800-34000 34000-68000
65-325
†
SNAP C18 12g* 15 12 <60 <504 <996 5-20
SNAP C18 30g* 33 30 <210 <1740 <3000 15-40
SNAP C18 60g 66 60 <540 <4500 <7020 25-50
SNAP C18 120g 132 120 <2040 <8040 <14040 25-50
SNAP C18 400g 510 400 <11200 <26000 <40000 50-100
SNAP NH 11g* 15 11 100-200 200-350 350-500 10-20
SNAP NH 28g* 33 28 250-500 500-875 875-1250 20-40
SNAP NH 55g 66 55 500-1000 1000-1750 1750-2500 50-100
SNAP NH 110g 132 110 1000-2000 2000-3500 3500-5000 50-100
SNAP NH 375g 510 375 3400-6800 6800-8900 8900-17000
65-300
†
Biotage ZIP 5g* 8 5 50-100 100-175 175-250 5-20
Biotage ZIP 10g* 15 10 100-200 200-350 350-500 10-20
Biotage ZIP 30g* 45 30 300-600 600-1050 1050-1500 20-40
Biotage ZIP 45g* 60 45 450-900 900-1575 1575-2250 50-100
Biotage ZIP 80g 102 80 800-1600 1600-2800 2800-4000 50-100
Biotage ZIP 120g 170 120 1200-2400 2400-4200 4200-6000 50-100
FLASH 75S
§
264 200 2000-4000 4000-7000 7000-10000
100-500
†
FLASH 75M
§
528 400 4000-8000 8000-14000 14000-20000
100-500
†
FLASH 75L
§
1056 800 8000-16000 16000-28000 28000-40000
100-500
†