Appendix A Applications
A - 4 100-413-016 Rev. C
Collagen: an excellent fragmentation.
Colletotrichum capsici spores: 5 ml with 6 million spores/ml completely
disrupts in 4 minutes.
Cortiscosteroid: particle size can be reduced to approximately 5 microns while
large volumes can be treated at the rate of approximately 30 ml/minute on a con-
tinuous flow basis.
Corynebacterium: completely disrupts in 5 minutes with 50% protein release
and excellent enzyme activity.
Crypstostroma corticale (maple bark spores): a concentrated solution
completely disrupts in14 minutes.
Cryptococcus laurentii: completely disrupts in 7 minutes with good protein
release and enzyme activity.
Crystal reduction: large crystals of an organic compound suspended in iso-
propanol can be reduced in diameter 10-40%.
Cyanidium caldarium: a concentrated solution completely disrupts in 6 min-
utes.
Dental plaques: 5 ml solution, concentration 1 to 10,000, low power setting,
53,500,000 organisms per ml were obtained in 45 seconds.
Desullovibrio vulgaris: less than 30 seconds of ultrasonic treatment resulted
in release of TCA enzymes.
Diplococcus: completely disrupts in 5 minutes.
DNA: breaks chains on low power instantly. Controlled degradation may be
obtained.
Dyes: excellent rapid dispersion and homogenization.
E. coli: 2 gm wet wt in 10 ml solution completely disrupts in 40 seconds. The
Sonifier Cell Disruptor has been used extensively in research on this organism.
Egg whites: can be reduced to a homogeneous pipettable solution in 15 seconds
on low power.
Ehrlich ascites: disrupt in a few seconds.
Electron microscopy: apertures are quickly cleaned.
Emulsions: 10 ml of most light mixtures become semi-permanent emulsions in
about 1 minute without emulsifiers. Average particle size is usually well under 1
micron. Sterile emulsions can be prepared by ultrasonic treatment for feeding to
germ-free animals.
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