250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 7
Lysossomes: released enzymes quickly.
Malaria prolozoa: fast, excellent disruption.
Maple bark spores: complete disruption in 14 minutes.
Measles: disruption of virus antigen clumps present in infected cells on low
power. Ultrasonic treatment increased antigen titer 4-8 fold.
Methanobacillus omelianskii: 1 gm cells wet wt/ml completely disinte-
grates in 2 minutes for assaying methane.
Microbacterium lacticum: ultrasonic treatment used for malate dehydroge-
nase extraction.
Micrococci: a 13 ml solution completely disrupts in 15 minutes.
Micrococcus lactiliticus: 75 ml of a 20% suspension was disintegrated in 15
minutes and a good yield of the enzyme Xanthine dehydrogenase extracted.
Mineral rock: excellent for cleaning surfaces between polishing stages.
Mitochondria: separates from cells without injury. Mitochondria themselves
can be broken with longer ultrasonic treatment. Inner membrane sub units also
isolated.
Muscle tissue: 1 gm homogenized in 4 minutes; heart muscle in 6 minutes.
Mycobacteria: a 20 ml growing media completely disrupts in 14 minutes.
Clumps break quickly. An immunological compound prepared.
Mycoplasma antibody: a suspension of Campo-W cells treated for 5 minutes
gave 12 lines with the sera in a gel diffusion test. The extract was estimated to con-
tain 12.75 mg protein per ml by Blaret reaction.
Myeloma tumor cells: complete disruption in 10 minutes, 30% disruption in
2 minutes.
Myleran: made colloidal suspension and dissolved in approximately 1 minute.
N. crassa: nucleus was isolated and purified from conidial extracts after 5 min-
utes treatment.
Naeglerigruberi: this free-living soil amoeba was treated ultrasonically to
release sub cellular infectious material.
Neurospora: 40 ml, 4 minutes, produced more protein than freeze thawing for
study of enzymatic synthesis of cystathionine.
Nocardia ostenodes: breaks clumps and disintegrates in less than 10 minutes.
Nucleoprotein: extracted from tissue. May be degraded selectively.
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