Appendix A Applications
A - 12 100-413-016 Rev. C
Toxin and antitoxin: one example of many: Toxin preparations of whole cell
lysate (WCL) of the Inaba serotype strain 569E of the classic biotype of cholera
vibrio were grown on 3% Bacto peptane agar and harvested in distilled water at
18 hours. The unwashed suspensions were solubilized ultrasonically, clarified by
centrifugation and the supernat freeze-dried for the titration of cholare toxin in the
rabbit ileal loop.
Toxoplasma gondii: can be separated from white blood cells without injury.
Transplantation antigens: were extracted from spleen, thymus and lymph
nodes.
Trichomonas foetus: Complete disruption in a few seconds
Triolein: Complete stable emulsion in 2 minutes.
Trypanosomes: concentrated 10 ml solution completely disrupts in 4 minutes.
Uterus muscle: a 1/5 gm, 3 cc solution completely disrupts in 3 minutes for
coenzyme Q determination.
Vaccines: numerous advantages such as, more antigenic material released than
usual and the production of vaccines not obtainable by classified methods.
Various bacilli: complete disruption in 3 minutes.
Vibrio comma: excellent disruption.
Virus extraction: excellent for experimental vaccines. Evidence of breakage
of virus/antibody bonds. Virus can be extracted without damage at low power, or
broken at high power.
Vitamin E: 30 seconds of ultrasonic treatment put material in solution with a
resultant permanent suspension.
W138 virus: cell-free V-2 virus obtained in 30 seconds using 6 ml of Veronal
buffer with W138 cells containing V-2 virus.
Yeast: complete disruption in 3-10 minutes. Complete disruption of 9 grams
pressed yeast in 18 ml buffer in 8 minutes. Protein release, 52 mg/ml from an aged
sample.
To Next Page
Loading...
