250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 5
Enterococcus: excellent disruption.
Erwina cartovara: complete disruption in 1-2 minutes depending on cell con-
centration.
Erythrocytes: disrupts in a few seconds.
Euglena gracilis: completely disrupts in a few seconds to isolate chloroplasts.
Eugoena: complete disruption in 12 minutes, 90% disruption in 8 minutes with
pigment released.
Fat extraction: fat can be emulsified without injuring tissue with proper power
selection. Lipid layer can be stripped from spores and mycobacteria.
Fibrin: complete suspension of.125 gm in 30 minutes.
Fish gill: complete disruption of 20 mg in 30 seconds.
Fish tissue: tissue homogenization for extractions and excellent particle size
reduction in 8 minutes per 10 gm.
Fluorocarbons: extended treatment time will break down particle size to well
under 1 micron and gives a fine homogenate.
Fossils: low power will clean debris from delicate fossils without injury. Micro
fossils such as pollen can be separated from rocks to help identify the geological
age of the strata. Removal of rock matrix.
Gamma globulin: the Sonifier Cell Disruptor was used to solubilize protein as one
of the steps in the biosynthesis of gamma globulin from rabbit spleen.
Gangliosides: immunochemical and structure studies used ultrasonic treatment
as one step.
Gastric mucosa: placing scrapings into a test tube and test tube into new
water-filled cup horn caps permits these cells to be separated and not broken.
Graphite molybdenum disulfide: an excellent dispersion of this lubricant
was made in a silicate binder.
Guanine: produces colloidal suspension in 1 minute.
Gymnodinium: solution completely disrupts in 6 minutes.
Haemophilus pertussis: preparation of successful immunological com-
plexes.
Heart muscle: 1 gm disintegrates in 6 minutes.
HeLa Cells: disruption to free virus in a few seconds without injury.
Hemphilus pertussis: an immunological compound prepared.
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