Appendix A Applications
A - 6 100-413-016 Rev. C
Herpes virus: may be quickly released without injury.
Histoplasma capsulatum: ultrasonic treatment for 7 minutes completely rup-
tured cells prepared by formalin fixation. Good enzyme activity was obtained.
Human serum proteins: ultrasonic treatment causes a reproducible change in
the electrophoretic behavior of normal human serum consisting of an increase in
material migrating in the x and b globulin zones with a reduction in the albumin
and y globulin fractions.
Hydrocortisone: smaller crystals were produced by ultrasonic treatment.
Hydrophilic vegetable gums: disperses and solubilizes hydrophilic vegeta-
ble gums in water; makes dispersions of added particulate matter.
Intracellular membrane: disruption and particle size reduction obtained in
30-60 seconds.
Isoenzymes: selectively activated with respect to time and intensity of treat-
ment.
Kidney: 1 gm disintegrates in 3 minutes.
Kidney stones: easily broken in seconds in vitro.
Klebsiella: excellent disruption.
L. arabinosis: complete disruption to free virus in 2 minutes without injury.
Lactobacillus: 0.5 gm in 15 ml completely disrupts in 11 minutes. Excellent
release of acetokinase.
Lenconostoc mesenteroides: ultrasonic treatment for 15 minutes using high
power for disruption.
Leukocyte Iysozyme activity in myelocytic leukemia: the cell suspen-
sion was ultrasonically treated and samples assayed for Iysozyme activity. The
Iysozyme concentration of the leukocytes ug /10
6
cells was determined.
Linoleic acid: made suspension in water in 30 seconds.
Liver tissue: 1 gm homogenizes in less than 1 minute.
Lung tissue: 1 gm homogenizes in 2 minutes.
Lymphacytis: complete disruption in 15 seconds.
Lymphocyte nuclei: complete disruption in 6 minutes.
Lymph gland: direct injection lymphography with a modified radiopaque
emulsion was obtained by ultrasonic treatment in a functional procedure produc-
ing lymphatic structure detail.
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