6 Operation
34 Pump Combinations Operation Manual, Version E
6.4 Calibration
ATTENTION
Before carrying out the calibration make sure that the system is free of air. Always calibrate the
system under your actual working conditions (flow rate, backpressure, column). After calibration carry
out the measurement again to check the current pump flow rate.
The pump flow rate can be influenced by the following factors:
• The length of the suction tubing.
• The location of the solvent vessel above or below the pump.
• The viscosity of the solvent.
• The backpressure of the chromatography column.
• When using a suction tubing of which the inner diameter is too small.
6.4.1 Calibration of the Pump Controller C-610
Requirements:
For the calibration of Pump Controller C-610 you need a graduated cylinder and a stopwatch.
Set the pump flow rate to 100 ml. Place the suction tubing into the graduated cylinder and start the
pump. After exactly one minute take the tubing out of the cylinder.
If the graduated cylinder contains exactly 100 ml, the pump is calibrated. If too little or too much
solvent is in the cylinder, the pump flow rate is accordingly too low or too high.
If this is the case, recalibrate the pump.
Proceed as follows:
Switch off the pump. Hold the START button pressed while you switch the pump on again. Continue
pressing the START button for another 3 seconds. The display will read “+ C 00”: You are now in
Calibration mode. Increase or decrease the flow rate using the selector switch. Press the STOP button
to confirm the new value.
The pump will now deliver less or more solvent, accordingly. Perform the one-minute measurement of
100 ml again to check the current pump flow rate.
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