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Eppendorf 5804 - Imbalance Calibration Procedure

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Centrifuge 5804/5804 R and 5810/5810 R
3 Error lists / Troubleshooting
3-28 Error lists/Troubleshooting
Imbalance calibration carried out by a service technician
START Program 10
The centrifuge determines the imbalance cut-off value of the individual rotors from all rotors in a rotor
group.
One angled rotor (W) and
one swing-bucket rotor (A) must be used from each rotor group. As a
rule, this should be carried out with the standard rotors.
Exception: Use (A) Rotor A-4-81 if avaliable.
Standard rotors: Centrifuge 5804 / 5804R (W) F34-6-38 and (A) Rotor A-4-44
Standard rotors: Centrifuge 5810 / 5810R (W) F34-6-38 and
(A) Rotor A-4-62
Prepare the weights: G1/G2/G3
It is possible to weigh the weights yourself using Eppendorf tubes. Filled 2.0 ml tubes (approx. 3.2 g) are
recommended. The weight of the tube must also be taken into consideration.
With swing-bucket rotors, these should be placed flat, loose and without an adapter on the bottom of
the swing-bucket. With the angled rotor F34-6-38, water should be pipetted directly into the empty
bores. MTP rotors can be loaded and then filled with one MTP each.
Procedure for imbalance calibration
1. Insert fixed-angle rotor (W). (Use the present rotor lid).
2. Call up the service program: Hold down both cursor keys and switching on the device.
Display : SE 1
3. Using the cursor key, call up SE 10
4. Insert the tube with G1.
5. Close lid. Start run: Start
6. Three calibration runs are performed automatically. The lid opens automatically.
7. Insert weight G2.
8. Close the lid and wait until three further calibration runs have been performed.
Lid opens automatically.
9. Insert weight G3. Close lid. Start run: Start (Centrifuge must show "Imbal" and switch off.)
10. Open lid: Open.
11. Remove fixed-angle rotor (W) and insert swing-bucket rotor (A).
12. Using the corresponding imbalance amounts G1, G2, G3, carry out the procedure described in
points 2. to 9
13. Switch off the device. Remove the imbalance weights and then switch on the centrifuge again as
normal.
Note:
An imbalance adjustment must be carried out every time the motor, PCB Control or PCB Imbalance is
replaced or when the rotor is inserted!
After the motor has been replacedl or when the centrifuge has not been used for a long period, it should
run for approximately five minutes at maximum speed in order to ensure
that the vibration mounts have the normal degree of hardness.
MSTM/0408

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