31
7 Programming
Denaturation
T=95.0
°
0:00:45
Programming: see page 30.
Annealing
T=61.0
°
0:00:45
– Press the key for options for the
temperature command (temperature- and
time increment, ramp, ramp increment,
gradient).
The cursor must be on the program line
number in front of the temperature
command.
– Press the , keys and confirm by pressing .
– Press the keys and confirm by pressing .
– Press to proceed to Gradient or Elongation.
Gradient
Possible with Mastercycler
®
gradient only.
G=10.0
°
– Enter the gradient,
press the , keys and
confirm by pressing .
Elongation
T=72.0
°
0:00:45
Select the command T.
– Press the , keys and
confirm by pressing .
– Press the keys and
confirm by pressing .
Cycle
GOTO 2 REP 19
– Select the GOTO command using
the key (it may be necessary
to press the key several times)
Confirm by pressing .
– Enter the line number for the
beginning of the program section
which is to be repeated.
Confirm by pressing .
Enter the number of repeats (REP).
Confirm by pressing .
Note:
The total number of cycles is
REP + 1.
FILES . . Edit UNNAMED
Edit Lid 105°
Load NOWAIT AUTO
Standard 1 T= 95.0° 0:02:00
New 2 T= 95.0° 0:00:45
Delete 3 T= 61.0° 0:00:45
UNNAMED + 0.0 +0:00
– –/22.0° 10:22:06
6
1
4
5
1
>0<
FILES . . Edit UNNAMED
Edit 1 T= 95.0° 0:02:00
Load 2 T= 95.0° 0:00:45
Standard 3 T= 61.0° 0:00:45
New + 0.0 +0:00
Delete R= 3.0°/s +0:0°/s
UNNAMED G= 10.0°
– –/22.0° 10:22:06
7
2
4
5
FILES . . Edit UNNAMED
Edit 2 T= 95.0° 0:00:45
Load 3 T= 61.0° 0:00:45
Standard + 0.0 +0:00
New R= 3.0°/s +0:0
Delete G= 10.0°
UNNAMED 4 T= 72.0° 00:00:45
– –/22.0° 10:22:06
FILES . . Edit UNNAMED
Edit 3 T= 61.0° 0:00:45
Load + 0.0 +0:00
Standard R= 3.0°/s +0:0
New G= 10.0°
Delete 4 T= 72.0° 00:00:45
UNNAMED 5 GOTO
❋❋❋
REP
❋❋❋
– –/22.0° 10:22:06
To Next Page
Loading...