ÄKTA start System Cue Card, 29-0240-42 AC 6
Column preparation
The Column preparation method is used for:
Preparing a new column
Equilibrating the column.
Note: Equilibrate columns before starting a new run.
Instruction
Immerse the buer inlet tubing into the buer used in
the chromatographic run.
Connect the column in the ow path.
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Operation from the Instrument Display
Starting a run
Chromatography runs can be performed either by running
predened methods, or by using manual operations.
Perform a method run
The following method types are available with ÄKTA start
to perform chromatography runs:
Quick start
Templates
User dened
Follow the workows below to run predened methods.
Operation from the Instrument Display
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Operation from UNICORN start
Operation from UNICORN start
Tighten all connections and pump DM water through
the system to check for leaks.
Tighten any leaking connector.
Removal from cold room
Switch o the instrument and disconnect power cable.
Loosen all connections to prevent them sticking when
the system returns to room temperature.
Allow the instrument to stabilize at room temperature.
Tighten all connections and pump DM water through
system to check for leaks.
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