GE akta start - Flow Charts and Diagrams; Detailed Flow Path Diagrams

ÄKTA start System Cue Card, 29-0240-42 AC 10
Elution Pump wash
Equilibration
100
50
3 CV 1.2 CV
Sample
%B
Fractionation/collection
IEX Step 1 ml/5 ml HiTrap - Ion Exchange Chromatography
The bound proteins are eluted in a single step using single
elution buer.
Re-equilibration
Wash unbound
Elution
Pump wash
Equilibration
100
50
5 CV 5 CV 5 CV 5 CV
Sample
%B
Fractionation/
collection
IEX Gradient 1 ml/5 ml HiTrap - Ion Exchange
Chromatography
The bound proteins are eluted using two buers with linear
increase in the concentration of buer B over a specied
time, followed by a step with 100% B concentration.
Re-equilibration
Wash unbound
Elution
Pump wash
Equilibration
100
50
5 CV 5 CV 5 CV 5 CV
Sample
%B
Fractionation/
collection
20 CV
Gradient 0 to 100%
B
GF 16/60 HiPrep - Gel Filtration Chromatography
The proteins are eluted in a single step using single
elution buer.
The column needs to be pre-equilibrated before the
start of the run.
•
•
Elution Pump wash
Equilibration
100
50
0.2 CV 1.2 CV
Sample
%B
Fractionation/collection
Flow charts
The active ow path is depicted in Green color.
Flow path details for ow of 100% buer A
through Outlet valve to waste
A.
Flow path details for ow of 100% buer B
through Outlet valve to waste
B.
Flow path details during gradient
operation at specied B concentration
C.
Flow path details, Outlet valve collection
Note: Fraction collector is disabled.
D.