GE akta start User Manual

ÄKTA start System Cue Card, 29-0240-42 AC 9
The pre-set run parameters for each technique are listed in the table below.
Parameter AC step
1 ml
HiTrap
AC step
5 ml
HiTrap
DS 5 ml
HiTrap
DS
53 ml
HiPrep™
IEX step
1 ml
HiTrap
IEX step
5 ml
HiTrap
IEX
gradient
1 ml
HiTrap
IEX
gradient
5 ml
HiTrap
GF
16/60
HiPrep
Column Volume
(ml)
1.0 5.0 5.0 53.0 1.0 5.0 1.0 5.0 120
Flow rate
(ml/min)
1.0 5.0 3.0 5.0 1.0 5.0 1.0 5.0 1.0
Pressure Limit
(MPa)
0.3 0.3 0.3 0.2 0.3 0.3 0.3 0.3 0.2
Save Result to
USB *
Yes Yes Yes Yes Yes Yes Yes Yes Yes
Sample From Pump Pump Pump Pump Pump Pump Pump Pump Pump
Sample Volume
(ml) **
0.1** 0.1** 0.1** 0.1** 0.1** 0.1** 0.1** 0.1** 0.1**
Equilibration
Volume (CV)
5.0 5.0 3.0 3.0 5.0 5.0 5.0 5.0 0.2
Wash Unbound
Volume (CV)
15.0 15.0 NA NA 10.0 10.0 10.0 10.0 NA
Elution Option Isocratic Isocratic Isocratic Isocratic Isocratic Isocratic Gradient Gradient Isocratic
Target B Conc
(%)
100 100 NA NA 100 100 100 100 NA
Elution Volume
(CV)
5.0 5.0 1.2 1.2 5.0 5.0 20.0 20.0 1.2
Hold at 100% B
(CV)
NA NA NA NA NA NA 5.0 5.0 NA
Re-Equilibration
Volume (CV)
5.0 5.0 NA NA 5.0 5.0 5.0 5.0 NA
Fractionation
Volume (ml)
1.0 3.0 0.5 3.0 1.0 3.0 1.0 5.0 4.0
* the results will be saved only if the USB memory stick is
inserted before opening the method.
** variable parameter
Note: The sample can be loaded only through Pump.
Quick start methods description
The “ready to run” methods available with ÄKTA start
are presented below. The length of each step or phase is
indicated on the diagrams as column volumes (CV).
Sample: variable volume to be set by the user.
Pump wash:
- for AC/IEX: 5 ml Buer B, then 5 ml of Buer A wash.
- for DS/GF: 5ml of Buer A wash.
AC Step 1 ml/5 ml HiTrap - Anity Chromatography
Commonly used for purication of tagged proteins, e.g.,
His tag, GST tag, Mab tagged proteins, etc.
The bound proteins are eluted in a single step using
single elution buer.
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•
Re-equilibration
Wash unbound
Elution
Pump wash
Equilibration
100
50
5 CV 15 CV 5 CV 5 CV
Sample
%B
Fractionation/
collection
DS 5 ml HiTrap/53 ml HiPrep - Desalting/Buer Exchange
Chromatography
The proteins are eluted in a single step using single
elution buer.
Make sure to load the recommended sample volume.
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•

GE akta start Specifications

General

Brand	GE
Model	akta start
Category	Laboratory Equipment
Language	English