2
Press OK. The switch valve turns to the inlet B port.
3
Draw buffer with the purge syringe until liquid enters the syringe.
4
Replace the purge tubing with the stop plug.
5
Stop the pump by pressing end and then OK.
Flushing the pump with 100% ethanol:
1
Put inlet tubing A1 in deionized water.
2
Run the pump at 40 ml/min for 1 min and press pause/cont.
3
Move inlet tubing A1 to 100% ethanol
4
Press pause/cont, run the pump for 10–20 s and press pause/cont.
5
Set the flow rate to 5 ml/min using the arrow buttons.
6
Press pause/cont, run the pump for at least 30 s and press pause/cont.
7
Move inlet tubing A1 to deionized water.
8
Press pause/cont and run the pump for 1 min.
9
Finish by pressing end and then OK.
Preparing the tubing and column
1
Put inlet tubing A1 in the binding buffer.
2
Put inlet tubing B in the elution buffer.
3
Put the three waste capillaries (brown color) from port 4 and 5 on the injection valve
and port NO on the fraction collector valve in waste.
4
Connect a column, for example a HisTrap™ HP 1 ml column, between port 1 on the
injection valve and the upper port of the UV flow cell. Use a suitable length of PEEK
tubing and 1/16" male connectors.
36 ÄKTAprime plus Operating Instructions 28-9597-89 AB
4 Operation
4.3 Preparations before start
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