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Leica DFC500 - Page 5

Leica DFC500
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Warren Lab JMW, v3 06/2013
5
Capture Format Moving color filter (Bayer mosaic filter) relative to the CCD when taking a
picture, which requires 2 green, 1 red and 1 blue images to be taken. Therefore, the filter must
move 4 times to get all 4 images. The default setting is 1360x1024, 4 shot co-site color. A larger
number of shots will result in better resolution images but the file size will be huge.
Input Options – A particular camera setting can be saved as a default setting:
Input options > configuration > create new default setting
A variety of pre-defined contrast settings are available, but these are not of much use. The option
exists to turn off the chip cooling, but do not do this. The chip will perform better if cooled,
though it does not have to be cooled when not in use. When the software is not open, the cooling
fan is turned off.
Histogram Show the signal display, which can be manually adjusted. However this results in
loss of information as reduces each color element from full 255 spectrum. You should not have a
reason to adjust the histogram.
Region of Interest Can crop by drawing a box and only acquiring the image within the box.
Can zoom focus to magnify an area to aid in focusing.
Check Color – this can be adjusted after doing a white balance.
Calibration Settings Calculated indicates that the scale-bar is chosen based on the microscope
specifications. For the stereomicroscope, this is automatically detected. For the petrographic
microscope, this must be manually selected to match the objective.
HDR Imaging HDR stands for high dynamic range. This takes multiple exposure images to
capture brightest and darkest segments of the image. Heath (JH Technologies) is supposed to
provide more information on this, as it is a recent upgrade.
Left Click on Image allows you to draw a box and brings up menu options. Can use to select a
white balance area or for zoom focusing.
Right side buttons:
Pan window – allows scrolling around when zoomed in; Zoom in/out; Fit to screen; 1:1 - restores
image to full resolution.
Scale-bar Can adjust parameters, location. Acquiring an image does not necessarily save the
image unless the box "Merge" is checked. This can be done in post-processing by selecting
Merge, which will either replace the image or save a second file.
Dual Image Browser Can either compare live image to saved image or compare two acquired
images.

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