What to do if no definite DF contrast is possible on Leica Microscope?
If no definite DF contrast is possible with your Leica Microscope, ensure that you are using a DF objective. If the objective aperture is too high (maximum 0.75/ 1.10), reduce it through the iris diaphragm on the objective. Check the condenser centering and open the aperture diaphragm completely.
General| Category | Microscope |
|---|---|
| Illumination | LED |
| Focus | Coaxial coarse and fine focus knobs |
| Eyepiece | 10x |
| Condenser | Abbe condenser with iris diaphragm |
| Magnification | 40x to 1000x |
| Power Supply | AC adapter |
| Objective Lenses | 4x, 10x, 40x, 100x |
| Objectives | 4x, 10x, 40x, 100x |
Explains the importance of reading user manuals and safety instructions for safe operation of the microscope.
Provides guidelines for proper installation, placement, and usage to avoid potential hazards.
Discusses ergonomic risks, infection transmission via eyepieces, and eye damage from illumination.
Outlines responsibilities for ensuring safe operation, maintenance, and qualified personnel.
Details the procedure for accurately centering the condenser for optimal illumination.
Explains the steps for bringing the specimen into sharp focus using coarse and fine adjustment knobs.
Describes how to set up the Koehler illumination system for optimal contrast and illumination.
Provides detailed steps for using immersion oil with high-magnification objectives.
Provides solutions for common issues with the microscope stand, focus, illumination, and image quality.
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