What to do if there is no light output in response to the source ON command (serial or TTL) on Leica Laboratory Equipment (LED5/LED8 only)?
If there is no light output, verify that the liquid light guide is inserted in the light output receptacle, the external gate jumper is inserted in the front panel, and the control key is inserted in the front panel and turned to the “on” position. These three interlocks must be closed.
Why are fluorescence signals unusually weak across all detection channels in Leica LED1?
Weak fluorescence in all detection channels is likely due to poor light transmission by the liquid light guide, the collimating adaptor, or another distal component of the microscope optical path.
What to do if I see a high, spatially uniform fluorescence background on Leica LED1?
Verify that the dichroic beamsplitter and emission bandpass filter in the microscope are compatible with the light engine excitation filter specifications shown on the certificate of conformance.
What to do if Leica LED1 Laboratory Equipment shows unusually weak fluorescence signals in a single detection channel (e.g. DAPI)?
Check that the dichroic beamsplitter and emission bandpass filter in the microscope are compatible with light engine excitation filter specifications shown on the certificate of conformance.
How to fix no response to TTL trigger commands on Leica Laboratory Equipment?
Ensure that TTL trigger inputs are polarity positive, and that serial ON/OFF controls (PC or Control POD) are set to OFF state.
What to do if Leica Laboratory Equipment has no response to TTL trigger commands (LED1/3)?
The light output must be turned ON by either the manual light output switch (LED1 and LED3) or by the Leica POD (LED1) BEFORE applying the TTL control signal to the shutter.
General| Light Source | LED |
|---|---|
| Type | LED Illuminator |
| Compatibility | Leica Microscopes |
| Illumination | Bright, even illumination |
| Power Supply | External power supply |
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