SpectraMax M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
0112-0115 F 43
Read the Cuvette
1. Insert the cuvette into the chamber, making sure that the clear
sides are to the left and right (facing the instrument). Do not
touch the clear surfaces of the cuvette.
2. Make sure the cuvette is completely seated in the chamber and
close the cuvette door.
3. If the cuvette contains a blank (typically this solvent contains
everything that the samples contain except for analyte), press
the key to acquire the reference reading from the cuvette.
The instrument automatically calibrates in less than two
seconds, closes the microplate drawer (if it is open), and reads
the cuvette according to the selected instrument settings.
4. If the cuvette contains a sample, touch the key to
acquire the sample reading from the cuvette.
5. When the reading is complete, remove the cuvette.
Read the Microplate
1. Insert the filled microplate into the drawer, matching well A1
with position A1 in the drawer. Make sure the microplate is flat
against the drawer bottom (for 6-, 12-, 24-, or 48-well
microplates) or against the adapter (if using top read for 96- or
386-well plates-see
Installing the Drawer Adapter on page 36
for more information).
2. You must have SoftMax Pro Software running on a computer
connected to the instrument.
Open a SoftMax Pro data file or protocol file that contains the
appropriate experiment settings for the plate read. Alternatively,
create new settings by selecting the Plate section in the SoftMax
Pro program and configuring the instrument using the Plate
>Settings dialog box.
Note: The underside of the microplate must be dry prior to placing it in
the drawer. If the microplate has fluid on the underside, dry it using a
paper towel (or equivalent) before placing it in the drawer.
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