Molecular Devices SpectraMax M5 User Manual

0112-0115 F 7
1
Description
Introduction
The SpectraMax
®
M3, M4, M5, and M5
e
Microplate Readers are a series
of dual-monochromator, multidetection, multi-mode instruments with a
triple-mode cuvette port and 6-well to 384-well microplate reading
capability. Detection modalities are shown in Table 1-1.
The optical performance is comparable to a top-of-the-line dedicated
spectrophotometer or spectrofluorometer with no trade-off between
instrument performance and the number of read modes.
The built-in cuvette port can be used for absorbance, fluorescence and
luminescence readings. Dual monochromators allow selection of any
absorbance wavelength between 200 nm and 1000 nm, and any
excitation wavelength between 250 nm and 850 nm for readings in
fluorescence intensity, time-resolved fluorescence (M4, M5, M5
e
models
only) or wavelength-selectable luminescence modes, and 400–750 nm
for readings in fluorescence polarization mode (M5, M5
e
models only).
Assays requiring a read in two or more modes can be combined and run
on the SpectraMax Multi-Mode Microplate Readers by issuing a single
command in SoftMax
®
Pro Software, Molecular Devices’ leading
microplate data acquisition and analysis software platform.
Table 1-1 SpectraMax
®
Multi-Mode Microplate Readers and Applicable
Modes
Modes
SpectraMax
M3
SpectraMax
M4
SpectraMax
M5
SpectraMax
M5
e
Absorbance X X X X
Fluorescence intensity X X X X
Fluorescence
polarization
XX
Time-resolved
fluorescence
XXX
Luminescence x x X X
Note: In this user guide, all references to SpectraMax Multi-Mode
Microplate Readers include the M3, M4, M5, and M5
e
models. When a
feature or capability applies to only certain readers, this exception is
noted.

Molecular Devices SpectraMax M5 Specifications

General

Brand	Molecular Devices
Model	SpectraMax M5
Category	Laboratory Equipment
Language	English