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Molecular Devices versamax - Conventions Used in this Manual; Glossary of Terms; Absorbance, A; Optical Density, OD

Molecular Devices versamax
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VERSAmax Tunable Microplate Reader Operator’s Manual vii
Conventions
Used in this
Manual
The names of keys that appear on the VERSAmax
control panel are shown in
boxed Helvetica type. Example:
[Drawer]
.
Italic and boldface type are used for emphasis. Examples:
“Press carefully to
engage,”
“Do not press down.”
A note provides information that will help you properly execute an
action or procedure.
Indicates an action or condition that could potentially dam-
age the instrument or one of its components or could result in loss of
data.
Indicates a situation that could result in potential injury to a
person working with the system.
Indicates a condition involving potentially infectious bio-
logical agents requiring that proper handling precautions be taken.
Glossary of
Terms
Absorbance, A
The amount of light absorbed by a solution. To measure absorbance accu-
rately, it is necessary to eliminate light scatter. In the absence of turbidity,
absorbance = optical density.
A = log (I
0
/I)
I
0
= incident light
I = transmitted light
In this manual, we use the terms absorbance and optical density
interchangeably.
Optical Density, OD
The amount of light passing through a sample to a detector relative to the
total amount of light available. Optical Density includes absorbance of the
sample plus light scatter from turbidity.
Transmittance, T
The ratio of transmitted light to the incident light.
T = I/I
0
%T = 100 T
NOTE:
!
CAUTION:
WARNING:
BIOHAZARD: