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Molecular Devices ImageXpress Confocal HT.ai User Manual

Molecular Devices ImageXpress Confocal HT.ai
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5084773 A
March 2021
ImageXpress Confocal HT.ai
High-Content Imaging System
User Guide

Table of Contents

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Molecular Devices ImageXpress Confocal HT.ai Specifications

General IconGeneral
BrandMolecular Devices
ModelImageXpress Confocal HT.ai
CategoryLaboratory Equipment
LanguageEnglish

Summary

Safety Information

Laser Safety

Critical safety information regarding the use of laser light sources.

Electrical Safety

Precautions to prevent electrical injuries and equipment damage.

Chemical and Biological Safety

Guidelines for safe handling of chemicals and biological materials.

Water Immersion Option Safety

Safety considerations specific to the water immersion option.

Chapter 1: ImageXpress Confocal HT.ai System

ImageXpress Confocal HT.ai System Features

Overview of the system's hardware components and capabilities.

MetaXpress Software Features

Description of the software's acquisition and analysis capabilities.

Theory of Operation

Explanation of the system's optical path and components.

Chapter 2: Using the ImageXpress Confocal HT.ai System

Starting the System

Step-by-step guide to powering on the instrument and starting the software.

Understanding Status Lights

Information on interpreting status lights on the instrument and external light source.

Shutting Down the System

Procedures for safely powering down the entire system.

Chapter 3: System Installation and Testing

Verifying Device Settings in the Meta Imaging Series Administrator Software

How to verify hardware and software configuration using the administrator software.

Verifying Camera Settings in the Meta Imaging Series Administrator Software

Steps to verify the camera driver installation and responsiveness.

Verifying and Backing Up Settings in the MetaXpress Software

How to check and back up magnification, illumination, and calibration settings.

Verifying the Laser Autofocus Sensor

Procedure to verify the laser autofocus sensor response using a bead plate.

Verifying the Plate Reference Point (A1 Center)

How to verify the plate reference point is correctly set in well A1.

Chapter 4: Preparing for Acquisition

Assay Design

Guidelines for designing a high-content screening assay, considering downstream analysis.

Plate Selection

Factors to consider when selecting plates for assays to ensure image quality.

Sample Preparation

Considerations for sample preparation to ensure optimal imaging results.

Plate Acquisition Settings

Overview of acquisition settings configurable in the MetaXpress software.

Chapter 5: Configuring Plate Acquisition Options

Accessing the Plate Acquisition Setup Dialog

How to open the primary dialog for configuring acquisition protocols.

Plate Acquisition Setup Dialog Layout

Overview of the main functions and areas of the setup dialog.

Configuring Objective and Camera Settings

Defining objective, camera settings, binning, and acquisition mode for protocols.

Configuring Plate Dimensions

Setting plate dimensions for accurate stage movements and autofocus.

Configuring Sites to Visit

How to specify the number and layout of sites to acquire per well.

Configuring Acquisition Options

Specifying autofocus, wavelengths, and series acquisition options for protocols.

Saving Plate Acquisition Protocols

How to save acquisition protocols to the database or as a file.

Running Plate Acquisition Protocols

Procedures for running configured acquisition protocols.

Chapter 6: Customizing the MetaXpress Software

Users and Groups in the Meta Imaging Series Administrator Software

How to define and configure settings for users and groups in the software.

Custom Toolbars and Taskbars

How to create and modify custom toolbars and taskbars.

Default Paths for Data

How to change default file paths for data folders for users.

Chapter 7: Expansion Solution Options

Environmental Control Option

How the environmental control option maintains an environment for live-cell imaging.

Transmitted Light Option

Functionality of the transmitted light option for brightfield and phase-contrast imaging.

Water Immersion Option

How the water immersion option enhances image quality.

Performing Time Lapse Experiments

Enabling and setting up time lapse imaging experiments.

Chapter 8: Maintenance

Maintenance Precautions

General precautions to avoid injury or damage during maintenance procedures.

Routine Maintenance

Basic routine maintenance procedures to ensure optimal operation.

Cleaning the Instrument

General tips and procedures for cleaning the instrument.

Replacing the Confocal Disk Box

How to replace the confocal disk box.

Filter Maintenance

How to change or add filters to the system.

Objective Maintenance

Procedures for maintaining and cleaning objectives.

Obtaining Support

Technical Support

How to contact Molecular Devices Technical Support for assistance.

Appendix A: Instrument Specifications

Instrument Dimensions

Physical dimensions of the instrument, including height, width, and length.

Appendix B: Compatible Objectives

Nikon Objectives Compatible with the ImageXpress Confocal HT.ai System and Settings

Table detailing compatible Nikon objectives, their specs, and plate compatibility.

Appendix C: Filter Specifications and Calibration Compatibility

Filter Wavelengths

Specifications for filters, including excitation, emission, and dichroic wavelengths.

Appendix D: Optional Parts and Accessories

List of Optional Parts and Accessories

Details of available optional parts and accessories for the system.

Appendix E: File Privileges for the MetaXpress Software

Software Administrator

Required read/write access for software administrators.

Standard Users

Required read-only access for standard users.

Appendix F: Robotic Plate Handling

Robot step-by-step protocol

The sequence of actions for robotic plate handling.

Verifying External Control Settings

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