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ImageXpress Confocal HT.ai High-Content Imaging System User Guide

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Transmitted Light Option

The ImageXpressTransmitted Light option is designed for brightfield and phase-contrast

transmitted-light imaging. In general terms, transmitted light is used to describe microscopy in

which light is transmitted from a source on one side of the specimen, with the objective on the

other side. Typically, the light is first passed through a condenser so high illumination is

focused on the specimen. For more information on phase contrast microscopy, visit the Nikon

website:

www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html

This section describes the following:

Transmitted Light Hardware, see below

Transmitted Light Software Configuration, see page 165

Setting Up Koehler Illumination, see page 167

Transmitted Light Phase Ring Alignment, see page 170

Performing Transmitted Light Experiments, see page 176

Replacing the Transmitted Light Bulb, see page 177

Transmitted Light Hardware

The ImageXpressTransmitted Light option consists of the following hardware components.

Nikon phase optics. Allows either phase contrast or brightfield imaging. See and Figure 7-

36.

Phase contrast objectives. Must be selected for phase contrast imaging.

White light lamp (halogen). The software controls the white light lamp (halogen) power.

The lamp bulb has a limited life and can be replaced. See Replacing the Transmitted Light

Bulb on page 177.

WARNING! The surface of the halogen lamp gets very hot during operation and

remains hot for up to 20 minutes after operation. Do not to touch the lamp surface

while it is hot.

Transmitted Light shutter. Controlled by the software. The shutter protects the sample

from the transmitted light when not imaging. The shutter window maintains the

environment of the sample. See Figure 7-37.

Phase alignment lens. Located in the emission filter wheel and is placed in position using

the MetaXpress software for the ImageXpress Confocal HT.ai system.

Hinge. Allows robotic access to the plate if necessary. See Figure 7-38.

ImageXpressSystems Power and Options Controller. Used to turn the option on and off

and control the illumination intensity using the MetaXpress software. It is shared with the

ImageXpressEnvironmental Control option when both options are installed.

Molecular Devices ImageXpress Confocal HT.ai User Manual

Questions and Answers: