Chapter 1 Microscopy Procedures
14
Chap. 1-2
Microscopy Procedures Phase Contrast Microscopy
3
Set the setting mode switch to the left side (operation mode).
4
Turn on the dia-illumination LED.
5
Lower the condenser slightly from the uppermost position.
6
Move the condenser turret to the [A: empty] position.
Turn the condenser turret until the [A: empty] symbol
comes to the front, and align the hole with the optical path.
CLAMP
TORQUE
PHASE CONTRAST
0.90 DRY
JAPAN
A
Moving the condenser turret
to the [A: empty] position
Aperture diaphragm of the condenser
Positioning the condenser turret at positions other
than [A: empty] deviates the aperture diaphragm
from the optical path.
7
Fully open the field diaphragm and aperture diaphragm.
8
Bring the 10x Ph objective (Ph1) into the optical path.
9
Place a specimen on the stage, and move the stage to bring the target into view.
10
Focus on the specimen. (→See Chapter 2 “2 Focusing on the Specimen (Vertical Stage Movement)” for
details)
11
Adjust the diopter. (→See Chapter 2, “4 Adjusting the Diopter” for details)
12
Adjust the interpupillary distance.
13
Focus and center the condenser. (→See Chapter 2, “5 Focusing and Centering the Condenser” for details)
A: empt
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