Nikon Eclipse E400 POL User Manual

3. Operation of Each Part
LE]
Use
of
optional
accessories
(Sold
Separately)
m:
Р-С5
Sénarmont
Сотрепѕаїог
ШИ
The P-CS Sénarmont compensator is used by inserting
into
the
slot
of
the
intermediate
tube
in
place
of
the
P-CL 1/47. & tint plate
It can be used to measure retardation up to 12. according
to the following procedure.
It
is
normal
to
bring
the
top
lens
of
the
condenser
into
the
optical
path
when
observing
at
a
magnification
of
10x
or
greater.
Measuring
the
retardation
or
evaluating
by
interference
color,
however.
stop
down
the
condenser
aperture
diaphragm
or
swing
out
the
top
lens
even
for
a
magnification
of
10x
or
higher
(with
the
aperture
diaphragm
fully
opened),
and
illuminate
with
light
that
is
as
parallel
to
the
optical
axis
as
possible.
1 Determination of Extinction Position
Rotate
the
stage
with
the
specimen
under
the
crossed
Nicols
to
find
the
direction
where
the
part of the specimen to be measured appears darkest
2 Determination of Subtraction Position
Rotate
the
stage
45?
from
the
extinction
position
to
the
diagonal
position.
Insert
the
P-CL.
1/47.
&
tint
plate
into
the
optical
path
and
confirm
that
the
interference
color
of
the
section
of
the
specimen
to
be
measured
changes
toward
the
lower
order
side.
If
the
interference
color
changes
toward
the
higher
order
side,
rotate
the
stage
another
90°.
3 Measurement
Place
the
GIF
filter
on
the
field
lens
and
replace
the
P-CL
1/47.
&
tint
plate
with
the
P-CS
Sénarmont
compensator.
Rotate
the
analyzer
so
that
the
section
of
the
specimen
to
be
measured
becomes
darkest.
When
the
rotation
angle
of
the
analyzer
at
that
time
is
taken
to
be
theta
(8)
degrees,
then
retardation
(R)
(nm)
is
determined
with
the
following
formula:
(4: wavelength used)
The value of à when using the GIF filter is 546 nm.
25

Nikon Eclipse E400 POL Specifications

General

Brand	Nikon
Model	Eclipse E400 POL
Category	Microscope
Language	English