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Nikon Eclipse E400 User Manual

Nikon Eclipse E400
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“>.
Problem
Cause
+
*
....
Countermeasures
Viewing
is
poor;
details
cannot
be
seen
clearty;
too
much
contrast;
too
little
contrast.
The
compensation
ring
on
an
objective
with
a
compensation
ring
has
not
been
adjusted.
Adjust
the
compensation
ring
according
to
the
cover
glass.
There
is
dirt
or
dust
on
the
lens,
condenser,
objective
or
specimen.
Clean
the
components.
(p.30)
Uneven
focus
The
revolving
nosepiece
has
not
been
rotated
until
it
has
clicked
into
place.
Turn
the
revolving
nosepiece
until
it
clicks
into
place.
The
specimen
is
not
secured
in
place
on
the
stage.
Install the
specimen
properly
on
the
specimen
holder
on
the
stage.
The
stage
has
been
installed
on
a
slant.
Install
the
stage
correctly.
(p.28)
The
revolving
nosepiece
has
not
been
rotated
until
it
has
clicked
into
place.
Turn
the
revolving
nosepiece
until
it
clicks
into
place.
The
specimen
is
not
secured
in
place
on
the
stage.
Install
the
specimen
properly
on
the
specimen
Image
flows
holder
on
the
stage.
The
condenser
is
not
centered.
Center
the
condenser.
(p.27)
The
stage
has
been
installed
on
a
slant.
Install
the
stage
correctly.
(p.28)
An
NCB11
filter
is
not
being
used.
Use
the
NCBII
filter.
(p.14)
Image
is
yellowish
The
lamp
voltage
is
too
low.
Image
is
too
bright
The
lamp
voltage
is
too
high.
Inadequate
illumination
(also
check
the
electrical
system
problems
and
countermeasures)
The
lamp
voltage
is
too
low.
Push
auto-photo
switch
and
then
adjust
the
brightness
through
the
ND
filter
combination.
(p.15)
The
aperture
diaphragm
is
stopped
down
too
far.
Open
the
diaphragm
to
a
suitable
size.
(p.16)
The
condenser
is
too
low.
Position
the
condenser
so
that
the
image
of
the
field
diaphragm
forms
properly
on
the
specimen.
(p.27)
Focusing
is
not
possible
with
high-power
objectives
The
specimen
is
placed
upside-down.
Affix
the
specimen
on
the
stage
with
the
cover
glass
facing
up.
The
cover
glass
is
too
thick.
Use
the
specified
type
of
cover
glass
(thickness:
0.17
mm).
The
specimen
is
placed
upside-down.
Affix
the
specimen
on
the
stage
with
the
cover
The
objective
strikes
the
glass
facing
up.
(p.15)
specimen
when
switching
The
cover
glass
is
too
thick.
Use
the
specified
type
of
cover
glass
from
a
low-power
objective
.
.
.
a
(thickness:
0.17
mm).
to
a
high-power
objective
The
diopter
adjustment
has
not
been
made.
Perform
the
diopter
adjustment.
(p.14)
The
difference
in
focus
The
diopter
adjustment
has
not
been
made.
Perform
the
diopter
adjustment.
(p.14)
when
the
objective
is
switched
is
large
33

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Nikon Eclipse E400 Specifications

General IconGeneral
TypeBiological Microscope
Optical SystemCFI60 Infinity Optical System
NosepieceQuadruple nosepiece
Magnification40x to 1000x
Power SupplyAC 100-240V, 50/60Hz
Lamp Power6V 30W
Eyepiece10x
Objective Lenses4x, 10x, 40x, 100x
StageMechanical stage
FocusCoaxial coarse and fine focusing
CondenserAbbe condenser, N.A. 1.25
Observation TubeBinocular
Stage Dimensions134mm
Interpupillary Distance Adjustment50-75mm
Diopter Adjustment+/- 5 diopters
IlluminationBuilt-in Koehler illumination

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